Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum

IOCAS-IR

	Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum
	LIU Yun ; SONG Xiuxian; HAN Xiaotian ; YU Zhiming
发表期刊	Acta Oceanologica Sinica
ISSN	0253-505X
	2013-06-15
出版年	2013
卷号	v.32 期号:06 页码:84-90
文献类型	CNKI期刊论文
摘要	To verify the feasibility of high-affinity nitrate transporter gene (Nrt2) as an indicator of nitrogen status, changes in the transcript levels of transcripts associated with phosphate starvation and different nitrate concentrations were studied using real-time quantitative reverse-transcription PCR (QRT-PCR) technology in batch cultures of Skeletonema costatum. The results show that compared with P-replete condition, P starvation could reduce the Nrt2 transcript levels apparently. Nrt2 transcript levels had a significant negative linear correlation with nitrate concentrations below 40 μmol/L. The results of 48 h short-term incubation experiment under different nitrate concentrations confirmed this correlation, and the following regression equation is built: y = 3.305x + 98.95, R2 = 0.988, where x represents nitrate concentrations (<40 μmol/L) and y represents the Nrt2 transcript levels.
关键词	high-affinity nitrate transporter gene, phosphate starvation, nitrate concentrations, real-time PCR, Skeletonema costatum
CNKI专辑号	A;
CNKI专辑名称	基础科学;
CNKI专题号	A006;
CNKI专题名称	生物学;
分类号	Q943
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	The National Basic Research Program of China(973Program)under contract No.2010CB428706 ; the National Natural Science Foundation of China for Creative Research Groups under contract No.41121064 ; the National High Technology Research and Development Program of China under contract No.2008AA09Z107
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/190851
专题	中国科学院海洋研究所
作者单位	1.KeyLaboratoryofMarineEcologyandEnvironmentalSciences,InstituteofOceanology,ChineseAcademyofSciences 2.GraduateSchooloftheChineseAcademyofSciences
推荐引用方式 GB/T 7714	LIU Yun,SONG Xiuxian,HAN Xiaotian,et al. Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum. 2013.