Exploring valid internal-control genes in Porphyra yezoensis(Bangiaceae) during stress response conditions

IOCAS-IR

	Exploring valid internal-control genes in Porphyra yezoensis(Bangiaceae) during stress response conditions
	Wang WL(王文磊); Wu XJ(吴晓洁); Wang C(王超); Jia ZJ(贾兆君); He LW(何林文); Wei YF(韦一凡); Niu JF(牛建峰); Wang GC(王广策)
发表期刊	Chinese Journal of Oceanology and Limnology
ISSN	0254-4059
	2014-07-15
出版年	2014
卷号	v.32 期号:04 页码:49-57
文献类型	CNKI期刊论文
摘要	To screen the stable expression genes related to the stress(strong light, dehydration and temperature shock) we applied Absolute real-time PCR technology to determine the transcription numbers of the selected test genes in P orphyra yezoensis, which has been regarded as a potential model species responding the stress conditions in the intertidal. Absolute real-time PCR technology was applied to determine the transcription numbers of the selected test genes in P orphyra yezoensis, which has been regarded as a potential model species in stress responding. According to the results of photosynthesis parameters, we observed that Y(II) and F v / F m were significantly affected when stress was imposed on the thalli of P orphyra yezoensis, but underwent almost completely recovered under normal conditions, which were collected for the following experiments. Then three samples, which were treated with different grade stresses combined with salinity, irradiation and temperature, were collected. The transcription numbers of seven constitutive expression genes in above samples were determined after RNA extraction and cDNA synthesis. Finally, a general insight into the selection of internal control genes during stress response was obtained. We found that there were no obvious effects in terms of salinity stress(at salinity 90) on transcription of most genes used in the study. The 18 S ribosomal RNA gene had the highest expression level, varying remarkably among different tested groups. RPS8 expression showed a high irregular variance between samples. GAPDH presented comparatively stable expression and could thus be selected as the internal control. EF-1α showed stable expression during the series of multiple-stress tests. Our research provided available references for the selection of internal control genes for transcripts determination of P. yezoensis.
关键词	constitutive expression gene housekeeping gene Porphyra yezoensis Ueda real-time quantitative PCR stress responding
CNKI专辑号	A;D;
CNKI专辑名称	基础科学;农业科技;
CNKI专题号	D052;
CNKI专题名称	水产和渔业;
分类号	S968.431
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	Supported by the National Natural Science Foundation of China(Nos.41176134,30830015) ; the Prospective Joint Research Project of Jiangsu Province(No.BY2011188) ; the National Marine Public Welfare Research Project(Nos.201105008-2,201105023-7) ; the National Basic Research Program of China(973 Program)(No.2011CB411908)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/190403
专题	中国科学院海洋研究所
作者单位	1.CollegeofMarineScienceandEngineering,TianjinUniversityofScienceandTechnology 2.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 3.NantongBranch,InstituteofOceanology,ChineseAcademyofSciences
推荐引用方式 GB/T 7714	Wang WL,Wu XJ,Wang C,et al. Exploring valid internal-control genes in Porphyra yezoensis(Bangiaceae) during stress response conditions. 2014.