A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance

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	A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance
	Liu JW(刘敬文); Yu Y(于洋); Li FH(李富花); Zhang XJ(张晓军); Xiang JH(相建海)
发表期刊	Chinese Journal of Oceanology and Limnology
ISSN	0254-4059
	2014-11-15
出版年	2014
卷号	v.32 期号:06 页码:35-50
文献类型	CNKI期刊论文
摘要	Anti-lipopolysaccharide factors(ALFs) are basic components of the crustacean immune system that defend against a range of pathogens.The cDNA sequence of a new ALF,designated nLvALF2,with an open reading frame encoding 132 amino acids was cloned.Its deduced amino acid sequence contained the conserved functional domain of ALFs,the LPS binding domain(LBD).Its genomic sequence consisted of three exons and four introns.nLvALF2 was mainly expressed in the Oka organ and gills of shrimps.The transcriptional level of nLvALF2 increased significantly after white spot syndrome virus(WSSV) infection,suggesting its important roles in protecting shrimps from WSSV.Single nucleotide polymorphisms(SNPs)were found in the genomic sequence of nLvALF2,of which 38 were analyzed for associations with the susceptibility/resistance of shrimps to WSSV.The loci g.2422 A>G,g.2466 T>C,and g.2529 G>A were significantly associated with the resistance to WSSV(P<0.05).These SNP loci could be developed as markers for selection of WSSV-resistant varieties of Litopenaeus vannamei.
关键词	anti-lipopolysaccharide factors(ALFs) single nucleotide polymorphisms(SNPs) white spot syndrome virus(WSSV) resistance Litopenaeus vannamei
CNKI专辑号	A;D;
CNKI专辑名称	基础科学;农业科技;
CNKI专题号	D052;
CNKI专题名称	水产和渔业;
分类号	S917.4
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	Supported by the National High Technology Research and Development Program of China(863 Program)(Nos.2012AA10A404,2012AA092205) ; the General Program of National Natural Science Foundation of China(Nos.31272683,31072203)to Dr.LI Fuhua
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/190254
专题	中国科学院海洋研究所
作者单位	1.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.UniversityofChineseAcademyofSciences 3.National&LocalJointEngineeringLaboratoryofEcologicalMariculture,InstituteofOceanology,ChineseAcademyofSciences
推荐引用方式 GB/T 7714	Liu JW,Yu Y,Li FH,et al. A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance. 2014.