Phylogenetic Analysis of Epibacterial Communities on the Surfaces of Four Red Macroalgae

IOCAS-IR

	Phylogenetic Analysis of Epibacterial Communities on the Surfaces of Four Red Macroalgae
	WU Hongqing ; LIU Min ; ZHANG Wuchang ; XIAO Tian
发表期刊	Journal of Ocean University of China
ISSN	1672-5182
	2014-12-06
出版年	2014
卷号	v.13 期号:06 页码:147-154
文献类型	CNKI期刊论文
摘要	Macroalgal surfaces are prone to being attached by bacteria. Epibacterial community structures on marine macroalgae are host-specific but temporally and spatially variable. In this study, we investigated the structure of epibacterial communities on the surfaces of four red macroalgae, Gracilaria lemaneiformis, Gloiopeltis furcata, Mazzaella sp. and Porphyra yezoensis, by analyzing the sequences of 16 S r RNA gene libraries. Healthy individuals of all macroalgae species were collected in winter from a farm at Dalian, China. The results showed that the epibacterial communities were mainly dominated by α-Proteobacteria, γ-Proteobacteria and Bacteroidetes. Deinococcus-Thermus, Spirochaetes and ε-Proteobacteria were also found. The majority of cloned sequences shared the greatest similarity to those of culturable organisms. A large portion of sequences from the α-Proteobacteria homed in Roseobacter clade, i.e., genera Ahrensia, Roseovarius, Litoreibacter, Octadecabacter, Thaiassobacter and Sulfitobacter, while members of Bacteroidetes mainly belonged to family Flavobacteriaceae. The cloned sequences could be separated into 66 OTUs at 0.01 distance value, and rare common OTUs were found among libraries. At genus level, Pseudoalteromonas dominated Gr. lemaneiformis and Gl. furcata libraries, accounting for 72.2% and 47.3%, respectively. Sulfitobacter dominated P. yezoensis library, accounting for 35.4%. A previously undefined cluster within Deinococcus-Thermus dominated Mazzaella sp. library, accounting for 24.6% of the all. These results indicated that a broad range of bacteria inhabited the surfaces of these macroalgae.
关键词	epibacterial community red alga 16S r RNA gene
CNKI专辑号	A;
CNKI专辑名称	基础科学;
CNKI专题号	A006;
CNKI专题名称	生物学;
分类号	Q949.29
语种	英文;
资助项目(基金）	supported by the Foundation for Innovative Research Groups of the National Natural Science Foundation of China (No. 41121064) ; the National High Technology Research and Development Program of China (863 Program) (No. 2007AA09Z434)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/190222
专题	中国科学院海洋研究所
作者单位	1.KeyLaboratoryofMarineEcologyandEnvironmentalSciences,InstituteofOceanology,ChineseAcademyofSciences(CAS) 2.SchoolofMarineScienceandEngineering,HebeiUniversityofTechnology 3.GraduateUniversityofChineseAcademyofSciences 4.KeyLaboratoryofBiologyandGeneticResourcesofTropicalCrops,InstituteofTropicalBioscienceandBiotechnology,ChineseAcademyofTropicalAgriculturalScience(CATAS)
推荐引用方式 GB/T 7714	WU Hongqing,LIU Min,ZHANG Wuchang,et al. Phylogenetic Analysis of Epibacterial Communities on the Surfaces of Four Red Macroalgae. 2014.