Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature

IOCAS-IR

	Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature
	Liu YF(柳意樊); Ma DY(马道远); Xiao ZZ(肖志忠); Xu SH(徐世宏); Wang YF(王彦丰); Wang YF(王雨福); Xiao YS(肖永双); Song ZC(宋宗诚); Teng ZJ(滕照军); Liu QH(刘清华); Li J(李军)
发表期刊	Chinese Journal of Oceanology and Limnology
ISSN	0254-4059
	2015-01-15
出版年	2015
卷号	v.33 期号:01 页码:13-21
文献类型	CNKI期刊论文
摘要	High temperature influences the homeostasis of fish.We investigated the effects of elevated temperature on tissues of Japanese flounder {Paralichthys olivaceus) by analyzing the histology and heat shock protein 70(hsp70) expression of fish reared in warm conditions.In this study,temperature was increased at l±0.5℃/day starting at 24±0.5℃,and was kept at that temperature for 5 days before the next rise.After raising temperature at the rate up to 32±0.5℃,tissue samples from midgut,spleen,stomach,liver,muscle,gill,heart,trunk kidney and brain were collected for histological analysis and mRNA assay.Almost all the tissues showed changes in morphological structure and hsp70 level at 32±0.5℃.Histological assessment of the tissues indicated that the gill had the most serious damage,including highly severe epithelial lifting and edema,curved tips and hyperemia at the ending of the lamellars,desquamation and necrosis.The next most severe damage was found in liver and kidney.The hsp70 levels in all the tissues first increased and then decreased.The gut,stomach,muscle,heart,and brain had the highest expressions in 6 h,whereas the spleen,liver,gill and kidney had the highest expressions in 2 h.Therefore,tissues with the most significant lesions(especially gill and liver) responded much earlier(2 h) in hsp70 expression than other tissues,and these tissues demonstrated the most marked histological disruption and elevated mRNA levels,making them ideal candidates for further studies on the thermal physiology of this species.
关键词	temperature Paralichthys olivaceus histology hsp70 tissue expression
CNKI专辑号	A;D;
CNKI专辑名称	基础科学;农业科技;
CNKI专题号	D052;
CNKI专题名称	水产和渔业;
分类号	S917.4
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	Supported by the Modern Agro-Industry Technology Research System of China(No.nycytx-50) ; the Key Innovation Program of Chinese Academy of Sciences,the Experiment and Demonstration of Scientific and Technical Innovation on Modern Ecological Ocean Agriculture(No.KSC2-EW-B-3) ; the Transformation Fund for Agricultural Science and Technology Achievements(No.2013GB2C600263) ; the Science Technology R&D Project of Shandong Province(No.2011GHy 11530) ; the Shandong Province Agricultural Seed Project(No.2014-2016) ; the Jiangsu Provincial Natural Science Foundation of China(No.BK2012222) ; the Fundamental Research Project of Technology Program of Qingdao,China(Nos.12-l-4-8(6)-jch,12-l-4-8-(7)-jch,12-4-1-51-hy)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/190162
专题	中国科学院海洋研究所
作者单位	1.InstituteofOceanology,ChineseAcademyofSciences 2.UniversityofChineseAcademyofSciences 3.NantongBranch,InstituteofOceanoIogy,ChineseAcademyofSciences 4.WeihaiShenghangAquaticProductScienceandTechnologyCo.Ltd. 5.RizhaoProliferationStationofMarineAquaticResources
推荐引用方式 GB/T 7714	Liu YF,Ma DY,Xiao ZZ,et al. Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature. 2015.