Isolation, Expression and Characterization of rbc L Gene from Ulva prolifera J. Agardh(Ulvophyceae, Chlorophyta)

IOCAS-IR

	Isolation, Expression and Characterization of rbc L Gene from Ulva prolifera J. Agardh(Ulvophyceae, Chlorophyta)
	SHAO Zhanru ; LI Wei ; GUO Hui ; DUAN Delin
发表期刊	Journal of Ocean University of China
ISSN	1672-5182
	2015-12-06
出版年	2015
卷号	v.14 期号:06 页码:161-169
文献类型	CNKI期刊论文
摘要	Ulva prolifera is a typical green alga in subtidal areas and can grow tremendously fast. A highly efficient Rubisco enzyme which is encoded by Up Rbc L gene may contribute to the rapid growth. In this study, the full-length Up Rbc L open reading frame(ORF) was identified, which encoded a protein of 474 amino acids. Phylogenetic analysis of Up Rbc L sequences revealed that Chlorophyta had a closer genetic relationship with higher plants than with Rhodophyta and Phaeophyta. The two distinct residues(aa11 and aa91) were presumed to be unique for Rubisco catalytic activity. The predicted three-dimensional structure showed that one α/β-barrel existed in the C-terminal region, and the sites for Mg~(2+)coordination and CO_2 fixation were also located in this region. Gene expression profile indicated that Up Rbc L was expressed at a higher level under light exposure than in darkness. When the culture temperature reached 35℃, the expression level of Up Rbc L was 2.5-fold lower than at 15℃, and the carboxylase activity exhibited 13.8-fold decrease. Up Rbc L was heterologously expressed in E. coli and was purified by Ni~(2+) affinity chromatography. The physiological and biochemical characterization of recombinant Rubisco will be explored in the future.
关键词	Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit sequence analysis real-time PCR in vitro expres-sion Ulva prolifera
CNKI专辑号	A;D;
CNKI专辑名称	基础科学;农业科技;
CNKI专题号	D052;
CNKI专题名称	水产和渔业;
分类号	S917.3
语种	英文;
资助项目(基金）	financially supported by the Scientific and Technical Supporting Programs of China(2008 BAC49B01) ; State Ocean Administration Project(200805069)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/189856
专题	中国科学院海洋研究所
作者单位	1.InstituteofOceanology,ChineseAcademyofSciences 2.InstituteofGeneralBotanyandPlantPhysiology,FriedrichSchillerUniversity 3.UniversityofChineseAcademyofSciences
推荐引用方式 GB/T 7714	SHAO Zhanru,LI Wei,GUO Hui,et al. Isolation, Expression and Characterization of rbc L Gene from Ulva prolifera J. Agardh(Ulvophyceae, Chlorophyta). 2015.