Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus macrocephalus

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	Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus macrocephalus
	Wang XY(王学颖); Shi XH(史雪辉); Liu YF(柳意樊); Yu DD(于道德); Guan SG(官曙光); Liu QH(刘清华); Li J(李军)
发表期刊	Chinese Journal of Oceanology and Limnology
ISSN	0254-4059
	2016-07-15
出版年	2016
卷号	v.34 期号:04 页码:137-145
文献类型	CNKI期刊论文
摘要	The present study evaluated the ef fects of chilled storage and cryopreservation on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod Gadus macrocephalus. Sperm motility and the activities of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx), glutathione reductase(Gr), and lipid peroxidation(measured via malondialdehyde(MDA) content) were determined after the milt was stored at 4°C for 12 h, cryopreserved without cryoprotectant in 12% propylene glycol(PG), cryopreserved in 12% PG+0.1 mol/L trehalose, or cryopreserved in 12% PG spermatozoa but centrifuged to decant the supernatant prior to cryopreservation(only sperm cells were cryopreserved). After chilled storage or cryopreservation, the SOD, CAT and GPx activities were reduced in sperm cells and increased in seminal plasma in almost all treatments; sperm motility parameters were also decreased. However, the addition of trehalose into the cryoprotectant could signifi cantly improve the postthaw sperm quality as revealed by the sperm average path velocity. This improvement might be attributed to the function of trehalose in scavenging reactive oxygen species. Chilled storage and cryopreservation had signifi cant eff ects on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod.
关键词	Pacific cod sperm seminal plasma cryopreservation sperm physiology
CNKI专辑号	A;D;
CNKI专辑名称	基础科学;农业科技;
CNKI专题号	D052;
CNKI专题名称	水产和渔业;
分类号	S917.4
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	Supported by the National Natural Science Foundation of China(Nos.31072212,41076100) ; the CAS Scientific and Technological Innovation Program for Cross and Cooperative Team,Marine Economy Innovative Development Project(No.12PYY001SF08) ; the National Key Basic Program of Science and Technology Platforms of Aquaculture Stock Resources,Shandong Technology Development Project(No.2013GHY11509)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/189605
专题	中国科学院海洋研究所
作者单位	1.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.CollegeofEarthScience,UniversityofChineseAcademyofSciences 3.CollegeofChemistryandPharmaceuticalSciences,QingdaoAgriculturalUniversity 4.MarineBiologyInstituteofShandongProvince 5.National&LocalJointEngineeringLaboratoryofEcologicalMariculture,ChineseAcademyofSciences
推荐引用方式 GB/T 7714	Wang XY,Shi XH,Liu YF,et al. Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus macrocephalus. 2016.