Structure-based function prediction of the expanding mollusk tyrosinase family

IOCAS-IR

	Structure-based function prediction of the expanding mollusk tyrosinase family
	Huang RL(黄荣莲); Li L(李莉); Zhang GF(张国范)
发表期刊	Chinese Journal of Oceanology and Limnology
ISSN	0254-4059
	2017-11-15
出版年	2017
卷号	v.35 期号:06 页码:196-206
文献类型	CNKI期刊论文
摘要	Tyrosinase(Ty) is a common enzyme found in many dif ferent animal groups. In our previous study, genome sequencing revealed that the Ty family is expanded in the Pacific oyster( Crassostrea gigas). Here, we examine the larger number of Ty family members in the Pacific oyster by high-level structure prediction to obtain more information about their function and evolution, especially the unknown role in biomineralization. We verified 12 Ty gene sequences from C rassostrea gigas genome and Pinctada fucata martensii transcriptome. By using phylogenetic analysis of these Tys with functionally known Tys from other molluscan species, eight subgroups were identified(Cg Ty_s1, Cg Ty_s2, Mol Ty_s1, Mol Ty-s2, Mol Ty-s3, Pin Ty-s1, Pin Ty-s2 and Pvi Ty). Structural data and surface pockets of the dinuclear copper center in the eight subgroups of molluscan Ty were obtained using the latest versions of prediction online servers. Structural comparison with other Ty proteins from the protein databank revealed functionally important residues(HA 1, H A2, H A3, H B1, H B2, H B3, Z1–Z9) and their location within these protein structures. The structural and chemical features of these pockets which may related to the substrate binding showed considerable variability among mollusks, which undoubtedly defines Ty substrate binding. Finally, we discuss the potential driving forces of Ty family evolution in mollusks. Based on these observations, we conclude that the Ty family has rapidly evolved as a consequence of substrate adaptation in mollusks.
关键词	tyrosinase mollusk ligand binding pocket substrate diversity evolution
CNKI专辑号	A;
CNKI专辑名称	基础科学;
CNKI专题号	A006;
CNKI专题名称	生物学;
分类号	Q55
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	Supported by the National Natural Science Foundation of China(No.31530079) ; the Western Pacifi c Ocean System:Structure,Dynamics and Consequences(No.XDA11000000) ; the Technological Innovation Project(No.2015ASKJ02-03,fi nancially supported by Qingdao National Laboratory for Marine Science and Technology) ; the Earmarked Fund for Modern Agro-Industry Technology Research System(No.CARS-48)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/189087
专题	中国科学院海洋研究所
作者单位	1.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.UniversityofChineseAcademyofSciences 3.MarinePearlCultureLab,FisheryCollege,GuangDongOceanUniversity 4.LaboratoryforMarineBiologyandBiotechnology,QingdaoNationalLaboratoryforMarineScienceandTechnology
推荐引用方式 GB/T 7714	Huang RL,Li L,Zhang GF. Structure-based function prediction of the expanding mollusk tyrosinase family. 2017.