Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity

IOCAS-IR

	Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity
	Sun YH(孙雨豪); Chen XL(陈晓琳); Liu S(刘松); Yu HH(于华华); Li RF(李荣锋); Wang XQ(王雪芹); Qin YK(秦玉坤); Li PC(李鹏程)
发表期刊	Journal of Oceanology and Limnology
ISSN	2096-5508
	2018-05-15
出版年	2018
卷号	v.36 期号:03 页码:284-293
文献类型	CNKI期刊论文
摘要	Heparin has been used as an anticoagulant drug for many years, but it has significant side ef fects. In the search for good substitutes, low molecular weight(MW) polysaccharides from S argassum fusiforme have been examined and confi rmed to possess biological activities. Here, S. fusiforme polysaccharides(SFP) were extracted and subjected to a hydrogen peroxide(H_2O_2) oxidation method for the preparation of low-MW SFP(LSFP). The effects of temperature, pH, and H_2O_2 concentration on the degradation process were also examined. Several LSFP of 36, 9, 5.7, and 2.7 kDa were obtained under different conditions, and their anticoagulant activities studied in vitro. The results showed that SFP and LSFP prolonged activated partial thromboplastin(APTT), prothrombin(PT) and thrombin times(TT) significantly, indicating that these low MW polysaccharides possessed anticoagulant activity in the intrinsic, extrinsic, and common coagulation pathways. As these ef fects were related to the MW of the polysaccharides in APTT and TT but not in PT, the contents of the monosaccharide fucose and sulfate and the polysaccharide MW could have exerted combined ef fects. The details of this mechanism require further verifi cation.
关键词	anticoagulant activity Sargassum fusiforme low molecular weight degradation
CNKI专辑号	A;B;
CNKI专辑名称	基础科学;工程科技Ⅰ辑;
CNKI专题号	B016;
CNKI专题名称	有机化工;
分类号	TQ464.1
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	Supported by the Ocean Public Welfare Scientifi c Research Project,State Oceanic Administration of the People’s Republic of China(No.201505033) ; the Qingdao People’s Livelihood Science and Technology Program(No.16-6-2-41-nsh)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/188936
专题	中国科学院海洋研究所
作者单位	1.InstituteofOceanology,ChineseAcademyofSciences 2.UniversityofChineseAcademyofSciences
推荐引用方式 GB/T 7714	Sun YH,Chen XL,Liu S,et al. Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity. 2018.