Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus

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	Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus
	Zhao CY(赵春彦); Xu SH(徐世宏); Feng CC(丰程程); Liu YF(柳意樊); Yang Y(杨阳); Wang YF(王彦丰); Xiao YS(肖永双); Song ZC(宋宗诚); Liu QH(刘清华); Li J(李军)
发表期刊	Journal of Oceanology and Limnology
ISSN	2096-5508
	2018-07-15
出版年	2018
卷号	v.36 期号:04 页码:334-347
文献类型	CNKI期刊论文
摘要	Turbots( Schophthalmus maximus), one of the most important economic marine flatfish species, fail to undergo final spawning and spermiation naturally under artificial farming conditions. In vertebrates, reproduction is regulated by the brain-pituitary-gonadal axis(BPG-axis), and gonadotropin releasing hormone(GnRH) is one of its key components. Therefore, to better understand the physiology of reproduction in the turbot, three of the genes encoding GnRH subtypes— sbGnRH, c GnRH-II and sGnRH —were cloned and sequenced by isolating the cDNA sequences. The localizations and patterns of expression of their mRNAs were also evaluated during seasonal gonadal development. All three mRNAs were expressed abundantly in the brain; sbGnRH and sGnRH mRNAs were also detected in the gonads and pituitary gland, and sbGnRH expression was much higher than that of sGnRH, indicating the critical role of sbGnRH in regulating the BPG-axis. Moreover, the brain expression patterns of sbGnRH and sGnRH mRNAs showed an increased trend during gonadal development, peaking in mature stages. This indicated the direct regulation of gonadal development by the GnRH system. In addition, c GnRH-II mRNA expression showed no significant variations, suggesting that c GnRH-II is not critically involved in the control of reproduction. Further, the mRNA abundances of the three GnRH forms in the breeding season were significantly higher than those in immature and post-breeding stages in all analyzed brain areas. Therefore, we propose that sbGnRH is the most important hormone for the regulation of reproduction in turbot via the BPG-axis. These results will help in better understanding the reproductive endocrine mechanisms of turbots and lay the groundwork for additional studies aimed at comparing the reproductive physiology of wild individuals with those raised under artificial conditions.
关键词	turbot GnRH brain gonad reproductive development
CNKI专辑号	A;D;
CNKI专辑名称	基础科学;农业科技;
CNKI专题号	D052;
CNKI专题名称	水产和渔业;
分类号	S917.4
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	Supported by the National Natural Science Foundation of China(Nos.31472264,31572602) ; the China Agriculture Research System(No.CARS-47) ; the Scientific and Technological Innovation Project Financially supported by Qingdao National Laboratory for Marine Science and Technology(Nos.2015ASKJ02,2015ASKJ02-03-03) ; the Youth Innovation Promotion Association CAS and Chinese Academy of Science and Technology Service Network Planning(No.KFJ-EWSTS-060)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/188881
专题	中国科学院海洋研究所
作者单位	1.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.UniversityofChineseAcademyofSciences 3.LaboratoryforMarineBiologyandBiotechnology,QingdaoNationalLaboratoryforMarineScienceandTechnology 4.WeihaiShenghangAquaticProductScienceandTechnologyCo.Ltd.
推荐引用方式 GB/T 7714	Zhao CY,Xu SH,Feng CC,et al. Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus. 2018.