Analysis of cultivable aerobic bacterial community composition and screening for facultative sulfate-reducing bacteria in marine corrosive steel

IOCAS-IR

	Analysis of cultivable aerobic bacterial community composition and screening for facultative sulfate-reducing bacteria in marine corrosive steel
	LI Xiaohong ; XIAO Hui ; ZHANG Wenjun ; LI Yongqian ; TANG Xuexi ; DUAN Jizhou; YANG Zhibo ; WANG Jing; GUAN Fang; DING Guoqing
发表期刊	Journal of Oceanology and Limnology
ISSN	2096-5508
	2019-03-15
出版年	2019
卷号	v.37 期号:02 页码:228-242
文献类型	CNKI期刊论文
摘要	Anaerobic, aerobic, and facultative bacteria are all present in corrosive environments. However,as previous studies to address corrosion in the marine environment have largely focused on anaerobic bacteria, limited attention has been paid to the composition and function of aerobic and facultative bacteria in this process. For analysis in this study, ten samples were collected from rust layers on steel plates that had been immersed in seawater for dif ferent periods(i.e., six months and eight years) at Sanya and Xiamen,China. The cultivable aerobic bacterial community structure as well as the number of sulfate-reducing bacteria(SRB) were analyzed in both cases, while the proportion of facultative SRB among the isolated aerobic bacteria in each sample was also evaluated using a novel approach. Bacterial abundance results show that the proportions are related to sea location and immersion time; abundances of culturable aerobic bacteria(CAB) and SRB from Sanya were greater in most corrosion samples than those from Xiamen,and abundances of both bacterial groups were greater in samples immersed for six months than for eight years. A total of 213 isolates were obtained from all samples in terms of CAB community composition,and a phylogenetic analysis revealed that the taxa comprised four phyla and 31 genera. Bacterial species composition is related to marine location; the results show that Firmicutes and Proteobacteria were the dominant phyla, accounting for 98.13% of the total, while Bacillus and Vibrio were the dominant genera,accounting for 53.06% of the total. An additional six facultative SRB strains were also screened from the isolates obtained and were found to encompass the genus Vibrio(four strains), Staphylococcus(one strain),and Photobacterium(one strain). It is noteworthy that mentions of Photobacterium species have so far been absent from the literature, both in terms of its membership of the SRB group and its relationship to corrosion.
关键词	marine corrosive steel cultivable aerobic bacteria facultative sulfate-reducing bacteria bacterial community composition 16S rRNA gene sequencing
CNKI专辑号	A;
CNKI专辑名称	基础科学;
CNKI专题号	A010;
CNKI专题名称	海洋学;
分类号	P755.3
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	Supported by the National Basic Research Program of China(973 Program)(No.2014CB643304) ; the National Natural Science Foundation of China(No.41576080) ; the Key Research and Development Program of Shandong Province(No.2018GHY115003)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/188616
专题	中国科学院海洋研究所
作者单位	1.CollegeofMarineLifeSciences,OceanUniversityofChina 2.KeyLaboratoryofMarineEnvironmentalCorrosionandBiofouling,InstituteofOceanology,ChineseAcademyofSciences 3.OpenStudioforMarineCorrosionandProtection,QingdaoNationalLaboratoryforMarineScienceandTechnology 4.NingboTianheAquaticEcosystemsEngineeringCo.Ltd. 5.QingdaoResearchInstituteforMarineCorrosion
推荐引用方式 GB/T 7714	LI Xiaohong,XIAO Hui,ZHANG Wenjun,et al. Analysis of cultivable aerobic bacterial community composition and screening for facultative sulfate-reducing bacteria in marine corrosive steel. 2019.