Bacterial communities fluctuate in abundance and diversity under simulated oil-contaminated seawater conditions

IOCAS-IR

	Bacterial communities fluctuate in abundance and diversity under simulated oil-contaminated seawater conditions
	LI Xiaohong ; YOU Cai ; QU Liang ; ZHOU Bin ; TANG Xuexi ; XIAO Hui
发表期刊	Journal of Oceanology and Limnology
ISSN	2096-5508
	2019-03-15
出版年	2019
卷号	v.37 期号:02 页码:243-255
文献类型	CNKI期刊论文
摘要	Marine bacteria have recently been identified as a potent solution for petroleum hydrocarbon degradation in response to hazardous oceanic oil spills. In this study, a mesocosm experiment simulating a petroleum spill event was performed to investigate changes in the abundance, structure, and productivity of bacterial communities in response to oil pollution. Cultured heterotrophic bacteria and total bacteria showed a consistent trend involving an immediate decrease in abundance, followed by a slight increase, and a steady low-level thereafter. However, the changing trend of bacterial productivity based on bacterial biomass and bacterial volume showed the opposite trend. In addition, the density of oil-degrading bacteria increased initially, then subsequently declined. The change in the bacterial community structure at day 0 and day 28 were also analyzed by amplified ribosomal DNA restriction analysis(ARDRA), which indicated that the species diversity of the bacterial community changed greatly after oil pollution. Alphaproteobacteria(40.98%)replaced Epsilonproteobacteria(51.10%) as the most abundant class, and Gammaproteobacteria(38.80%)became the second most dominant class in the whole bacterial community. The bacterial communities in oil-contaminated seawater(32 genera) became much more complex than those found in the natural seawater sample(16 genera). The proportion of petroleum-degrading bacteria in the oil-contaminated seawater also increased. In this study, culture-dependent and culture-independent approaches were combined to elucidate changes in both bacterial productivity and community structure. These findings will contribute to a better understanding of the role that bacteria play in material cycling and degradation in response to oil pollution.
关键词	petroleum pollution bacterial community bacterial growth amplified ribosomal DNA restriction analysis(ARDRA)
CNKI专辑号	A;B;
CNKI专辑名称	基础科学;工程科技Ⅰ辑;
CNKI专题号	A006;A010;B027;
CNKI专题名称	生物学;海洋学;环境科学与资源利用;
分类号	X55;X172
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	Supported by the NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences(No.U1606404) ; the Fundamental Research Funds for the Central Universities(No.201562018) ; the Foundation of the Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology(No.201108)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/188611
专题	中国科学院海洋研究所
作者单位	1.CollegeofMarineLifeSciences,OceanUniversityofChina 2.LaboratoryforMarineEcologyandEnvironmentalScience,QingdaoNationalLaboratoryforMarineScienceandTechnology 3.InstituteofOceanology,ChineseAcademyofSciences 4.CNOOCLimitedTianjinBranch
推荐引用方式 GB/T 7714	LI Xiaohong,YOU Cai,QU Liang,et al. Bacterial communities fluctuate in abundance and diversity under simulated oil-contaminated seawater conditions. 2019.