Genetic analysis of selected Sargassum fusiforme (Harvey) Setchell (Sargassaceae, Phaeophyta) strains with RAPD and ISSR markers

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	Genetic analysis of selected Sargassum fusiforme (Harvey) Setchell (Sargassaceae, Phaeophyta) strains with RAPD and ISSR markers
	YAO Jianting ; SHUAI Li ; LI Shengyao ; XU Caolu ; WANG Xiuliang
发表期刊	Journal of Oceanology and Limnology
ISSN	2096-5508
	2019-05-15
出版年	2019
卷号	v.37 期号:03 页码:7-13
文献类型	CNKI期刊论文
摘要	Since the 1980s, Sargassum fusiforme has been cultivated in Zhejiang, South China, and nowadays it becomes one of the important commercial seaweeds in China. With traditions of eating habits in the East Asian countries, this brown alga is used as food, because it contained functional oligo/polysaccharides and chemical components, and was regarded playing roles in antioxidant activities and regulating immunology. Through over 15 years' selection, breeding and cultivation, we obtained three strains with good traits and testified their characters during the production, which included the cultivars with high yield and other two good characters, either all the selected strains were applied in the Sargassum production. To avoid confusion during the selection and nursery, it was preferred to establish one fingerprint for distinguishing the Sargassum cultivars from different strains. Random amplified polymorphic DNA(RAPD) and inter-simple sequence repeat(ISSR) methods were adopted to analyze the genetic diversities of the selected S. fusiforme strains. With that, one fingerprint with RAPD markers was constructed, and one sequence characterized amplified region(SCAR) marker to S.fusiforme was obtained. It is indicated that the applied fingerprint could be valid in S. fusiforme genetic and germplasm justification, and will be positive to molecular marker assistance in its selection and cultivation.
关键词	Sargassum fusiforme random amplified polymorphic DNA(RAPD) inter-simple sequence repeat(ISSR) sequence characterized amplified region(SCAR) genetic analysis
CNKI专辑号	A;D;
CNKI专辑名称	基础科学;农业科技;
CNKI专题号	D052;
CNKI专题名称	水产和渔业;
分类号	S917.3
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	Supported by the Doutou Sci-Tech Project(No.N2006Y11B) ; the Shandong Key Sci-Technology Research Project(Nos.2018SDKJ0302-2,2018SDKJ0502-1) ; the CAS-Fujian STS Project(No.2017T3012)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/188566
专题	中国科学院海洋研究所
作者单位	1.KeyLabofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.LaboratoryforMarineBiologyandBiotechnology,QingdaoNationalLaboratoryforMarineScienceandTechnology 3.CenterforOceanMega-Science,ChineseAcademyofSciences 4.SchoolofEnvironmentalScienceandEngineering,QingdaoUniversity 5.DongtouYangqicaiResearchInstitute
推荐引用方式 GB/T 7714	YAO Jianting,SHUAI Li,LI Shengyao,et al. Genetic analysis of selected Sargassum fusiforme (Harvey) Setchell (Sargassaceae, Phaeophyta) strains with RAPD and ISSR markers. 2019.