Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells

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	Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells
	ZHOU Shengnan ; LIU Ge ; WU Shimei
发表期刊	Journal of Oceanology and Limnology
ISSN	2096-5508
	2020-05-15
出版年	2020
卷号	v.38 期号:03 页码:237-244
文献类型	CNKI期刊论文
摘要	Marine bacterial strain Bacillus sp. CS30 exhibited high anticancer activity against Huh7.5 human liver cancer. We purified the corresponding anticancer agent by sequential acidic precipitation, methanol extraction, Sephadex LH-20 chromatography, and reversed phase high-performance liquid chromatography(RP-HPLC), then analyzed it in mass spectrometry. Based on the results of purification and mass spectrometry, we deduced that the anticancer agent was the same component as our previously purified antifungal agent surfactin CS30-2. However, to the best of our knowledge, this is the first report on the surfactin possessing both antifungal and anticancer activities. Surfactin CS30-2 was demonstrated to exhibit high anticancer activity in a dose-dependent manner against Huh7.5 liver cancer cells. Further investigation showed that surfactin CS30-2 induced the increased generation of reactive oxygen species(ROS) and severe disruption of cell membrane, thus leading to cell death. However, unlike previously reported surfactins, surfactin CS30-2 caused cancer cell death via necrosis instead of apoptosis.
关键词	Bacillus surfactin anticancer activity reactive oxygen species(ROS)
CNKI专辑号	A;E;
CNKI专辑名称	基础科学;医药卫生科技;
CNKI专题号	E072;
CNKI专题名称	肿瘤学;
分类号	R735.7
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	Supported by the National Natural Science Foundation of China (No. 31600035) to WU Shimei
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/188231
专题	中国科学院海洋研究所
作者单位	1.CollegeofLifeSciences,QingdaoUniversity 2.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 3.LaboratoryforMarineBiologyandBiotechnology,QingdaoNationalLaboratoryforMarineScienceandTechnology
推荐引用方式 GB/T 7714	ZHOU Shengnan,LIU Ge,WU Shimei. Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells. 2020.