PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan

IOCAS-IR

	PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan
	YANG Yue; XING Rong'e ; LIU Song ; QIN Yukun; LI Kecheng ; YU Huahua; LI Pengcheng
发表期刊	Journal of Oceanology and Limnology
ISSN	2096-5508
	2020-05-15
出版年	2020
卷号	v.38 期号:03 页码:245-251
文献类型	CNKI期刊论文
摘要	We previously demonstrated that 2-hydroxypropyltrimethyl ammonium chloride chitosan(HACC) promoted the production of nitric oxide(NO) and proinflammatory cytokines by activating the mitogen-activated protein kinases(MAPK) and Janus kinase(JAK)/STAT pathways in RAW 264.7 cells, indicating good immunomodulatory activity of HACC. In this study, to further investigate the immunomodulatory mechanisms of HACC, we determined the roles of phosphatidylinositol 3-kinase(PI3 K)/Akt, activating protein(AP-1) and nuclear factor kappa B(NF-κB) in HACC-induced activation of RAW 264.7 cells by the western blotting. The results suggest that HACC promoted the phosphorylation of p85 and Akt. Furthermore, c-Jun and p65 were also increased after the treatment of RAW 264.7 cells with HACC, indicating the translocation of NF-κB and AP-1 from cytoplasm to nucleus. In addition, as scanning electron microscopy(SEM) analysis shows, the cell morphology changed after HACC treatment. These findings indicate that HACC activated MAPK, JAK/STAT, and PI3 K/Akt signaling pathways dependent on AP-1 and NF-κB activation in RAW 264.7 cells, ultimately leading to the increase of NO and cytokines.
关键词	hydroxypropyltrimethyl ammonium chloride chitosan RAW 264.7 cells PI3K/Akt pathway nuclear factor-κB activating protein 1
CNKI专辑号	A;
CNKI专辑名称	基础科学;
CNKI专题号	A006;
CNKI专题名称	生物学;
分类号	Q939.91
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	Supported by the National Key R&D Program of China (No. 2018YFC0311305) ; the Key Research and Development Program of Shandong Province (Nos. 2019GHY112015,2019YYSP028)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/188226
专题	中国科学院海洋研究所
作者单位	1.KeyLaboratoryofExperimentalMarineBiology,CenterforOceanMega-Science,InstituteofOceanology,ChineseAcademyofSciences 2.LaboratoryforMarineDrugsandBioproductsofQingdaoNationalLaboratoryforMarineScienceandTechnology 3.UniversityofChineseAcademyofSciences
推荐引用方式 GB/T 7714	YANG Yue,XING Rong'e,LIU Song,et al. PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan. 2020.