Nutrient depletion is the main limiting factor in the crude oil bioaugmentation process

IOCAS-IR

	Nutrient depletion is the main limiting factor in the crude oil bioaugmentation process
	Yanyu Sun ; Weiwei Chen ; Yibo Wang ; Jie Guo ; Haikun Zhang ; Xiaoke Hu
发表期刊	Journal of Environmental Sciences
ISSN	1001-0742
	2020-12-22
出版年	2021
卷号	v.100 期号:02 页码:319-329
文献类型	CNKI期刊论文
摘要	The biodegradation was considered as the prime mechanism of crude oil degradation. To validate the efficacy and survival of the crude oil-degrading strain in a bioremediation process, the enhanced green fluorescent protein gene( egfp) was introduced into Acinetobacter sp. HC8–3 S. In this study, an oil-contaminated sediment microcosm was conducted to investigate the temporal dynamics of the physicochemical characterization and microbial community in response to bacterium amendment. The introduced strains were able to survive, flourish and degrade crude oil quickly in the early stage of the bioremediation. However, the high abundance cannot be maintained due to the ammonium(NH 4 +-N) and phosphorus(PO 4 3--P) contents decreased rapidly after 15 days of remediation. The sediment microbial community changed considerably and reached relatively stable after nutrient depletion. Therefore, the addition of crude oil and degrading cells did not show a long-time impact on the original microbial communities, and sufficient nitrogen and phosphorus nutrients ensures the survive and activity of degrader. Our studies expand the understanding of the crude oil degradative processes, which will help to develop more rational bioremediation strategies.
关键词	Crude oil bioaugmentation Bacterial community dynamic shift Enhanced green fluorescent protein
CNKI专辑号	B;A;
CNKI专辑名称	工程科技Ⅰ辑;基础科学;
CNKI专题号	A006;B027;
CNKI专题名称	生物学;环境科学与资源利用;
分类号	X172
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	supported by the International Cooperation, Chinese Academy of Sciences, Chinese-foreign Coop-eration in key projects “The detection of oil spill and its ecological impact study” (No.133337KYSB20160002) ; “the State’s Key Project of Research and Development Plan” (No. 2016YFC1402300)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/187912
专题	中国科学院海洋研究所
作者单位	1.KeylaboratoryofCoastalBiologyandBioresourceUtilization,YantaiInstituteofCostalZoneResearch,ChineseAcademyofSciences 2.LaboratoryforMarineBiologyandBiotechnology,QingdaoNationalLaboratoryforMarineScienceandTechnology 3.CentreforOceanMega-Science,ChineseAcademyofSciences 4.UniversityofChineseAcademyofSciences
推荐引用方式 GB/T 7714	Yanyu Sun,Weiwei Chen,Yibo Wang,et al. Nutrient depletion is the main limiting factor in the crude oil bioaugmentation process. 2020.