Complementary DNA sequencing(cDNA):an effective approach for assessing the diversity and distribution of marine benthic ciliates along hydrographic gradients

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	Complementary DNA sequencing(cDNA):an effective approach for assessing the diversity and distribution of marine benthic ciliates along hydrographic gradients
	Pingping HUANG; Feng ZHAO ; Kuidong XU
发表期刊	Journal of Oceanology and Limnology
ISSN	2096-5508
	2021-01-15
出版年	2021
卷号	v.39 期号:01 页码:210-224
文献类型	CNKI期刊论文
摘要	The Yellow Sea Cold Water Mass(YSCWM) is a distinct hydrographic phenomenon of the Yellow Sea,and the distribution pattern of meio-and macrobentho s diffe rs inside and outside of the YSCWM.However,such a pattern has never been observed in the microbenthic ciliate communities.Therefore,we hypothesized that benthic ciliates followed a similar distribution pattern as meio-and macrobentho s,but this pattern has not been uncovered by morphological methods.We evaluated the diversity and distribution of benthic ciliates at five stations along hydrographic gradients across the YSCWM and adj acent shallow water by using morphology and DNA and complementary DNA(cDNA) high-throughput sequencing of the V4 region of 18 S rRNA gene.Results showed that the diversity of benthic ciliate s detected by DNA(303 OTU s),and the cDNA(611 OTUs) sequencing was much higher than that detected by the morphological method(79 species).Morphological method detected roughly different ciliate communities inside and outside of the YSCWM,but without statistical significance.No clear pattern was obtained by DNA sequencing.In contrast,cDNA sequencing revealed a distinct distribution pattern of benthic ciliate communities like meioand macrobenthos,which coincided well with the results of the environmental parameter analysis.More than half of the total sequences detected by DNA sequencing belonged to planktonic ciliates,most(if not all) of which were recovered from historic DNA originating through the sedimentation of pelagic forms because none of them were observed morphologically.The irrelevant historic DNA greatly influenced the recovery of rare species and thus limited the understanding of the benthic ciliate diversity and distribution.Our research indicates that the methods used have significant effects on the investigation of benthic ciliate communities and highlights that cDNA sequencing has great advantages in estimating the diversity and distribution of benthic ciliates,as well as the potential for benthic environmental assessments.
关键词	benthic ciliates cDNA high-throughput sequencing community comparison DNA high-throughput sequencing morphology
CNKI专辑号	A;
CNKI专辑名称	基础科学;
CNKI专题号	A006;
CNKI专题名称	生物学;
分类号	Q958.8
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	Supported by the National Natural Science Foundation of China (Nos. 41876171, 41506167, 41476144)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/187896
专题	中国科学院海洋研究所
作者单位	1.LaboratoryofMarineOrganismTaxonomyandPhylogeny,InstituteofOceanology,ChineseAcademyofSciences 2.LaboratoryforMarineBiologyandBiotechnology,PilotNationalLaboratoryforMarineScienceandTechnology(Qingdao) 3.CenterforOceanMega-Science,ChineseAcademyofSciences 4.UniversityofChineseAcademyofSciences
推荐引用方式 GB/T 7714	Pingping HUANG,Feng ZHAO,Kuidong XU. Complementary DNA sequencing(cDNA):an effective approach for assessing the diversity and distribution of marine benthic ciliates along hydrographic gradients. 2021.