Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa

IOCAS-IR

	Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa
	Qingchun ZHANG ; Zhuang NIU ; Jinxiu WANG; Chao LIU ; Fanzhou KONG; Xiaokun HU ; Jiayu ZHAO; Rencheng YU
发表期刊	Journal of Oceanology and Limnology
ISSN	2096-5508
	2021-03-15
出版年	2021
卷号	v.39 期号:02 页码:125-153
文献类型	CNKI期刊论文
摘要	Phaeocystis globosa is an important harmful algal bloom causative species distributing widely in temperate and tropical coastal waters in the world.The morphological,physiological,and biochemical characte ristics are different amo ng geographic strains,which can not be distinguished with nuclear ribo somal DNA markers at present.Therefore,the genetic distance and phylogeographic relationships of nuclear 28 S rDNA D1-D2 and ITS regions,and three chloroplast intergenic spacers(petN-trnS1,trnM1-psbA,and rbcS-rpl27) were analyzed and compared among 13 strains of P.globosa isolated from the Pacific Ocean and Atlantic Ocean in this study.In addition,the nucleotide polymorphisms of 28 S rDNA D1-D2,ITS,and rbcS-rpl27 regions were evaluated in two P.globosa strains.The various levels of nucleotide polymorphism were in the nuclear 28 S rDNA D1-D2 region and ITS region,but no polymorphism was in the chloroplast rbcS-rpl27 intergenic spacer.A reasonable intraspecific phylogeographic relationship was presented by rbcS-rpl27 intergenic spacer,which had the strongest distinction to geographic strains compared to those of 28 S rDNA D1-D2 and ITS regions.In the phylogenetic tree of rbcS-rpl27 intergenic spacer,the two strains from the North Sea of the Atlantic Ocean were divided firstly from the species of P.globosa,and then formed an independent clade,while the other Atlantic strains and all of Pacific strains j oined up to build the other clade.It was implied that at least two genetically distant populations of P.globosa existed in the Atlantic coastal regions.This study provided a high-resolution chloroplast marker to analyze intraspecific phylogeographic populations of P.globosa,and preliminarily clarified the genetic relationships of the Pacific and Atlantic strains of P.globosa.
关键词	Phaeocystis globosa chloroplast DNA marker phylogeny
CNKI专辑号	A;
CNKI专辑名称	基础科学;
CNKI专题号	A006;
CNKI专题名称	生物学;
分类号	Q948
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	the National Key Research and Development Plan Project ; the Sino-Australian Centre for Healthy Coasts (No. 2016YFE0101500) ; the Science&Technology Basic Resources Investigation Program (No. 2018FY100206) ; the National Natural Science Foundation of China (Nos. 41576121, 41776127)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/187825
专题	中国科学院海洋研究所
作者单位	1.CASKeyLaboratoryofMarineEcologyandEnvironmentalSciences,InstituteofOceanology,ChineseAcademyofSciences 2.LaboratoryforMarineEcologyandEnvironmentalSciences,PilotNationalLaboratoryforMarineScienceandTechnology(Qingdao) 3.CenterofOceanMega-Science,ChineseAcademyofSciences 4.UniversityofChineseAcademyofSciences
推荐引用方式 GB/T 7714	Qingchun ZHANG,Zhuang NIU,Jinxiu WANG,et al. Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa. 2021.