Smyd1 is essential for myosin expression and sarcomere organization in craniofacial, extraocular, and cardiac muscles

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	Smyd1 is essential for myosin expression and sarcomere organization in craniofacial, extraocular, and cardiac muscles
	Shuang Jiao ; Rui Xu; Shaojun Du
发表期刊	Journal of Genetics and Genomics
ISSN	1673-8527
	2021-03-20
出版年	2021
卷号	v.48 期号:03 页码:40-50
文献类型	CNKI期刊论文
摘要	Skeletal and cardiac muscles are striated myofibers that contain highly organized sarcomeres for muscle contraction. Recent studies revealed that Smyd1, a lysine methyltransferase, plays a key role in sarcomere assembly in heart and trunk skeletal muscles. However, Smyd1 expression and function in craniofacial muscles are not known. Here, we analyze the developmental expression and function of two smyd1 paralogous genes, smyd1 a and smyd1 b, in craniofacial and cardiac muscles of zebrafish embryos. Our data show that loss of smyd1 a(smyd1a~(mb5)) or smyd1 b(smyd1b~(sa15678)) has no visible effects on myogenic commitment and expression of myod and myosin heavy-chain m RNA transcripts in craniofacial muscles.However, myosin heavy-chain protein accumulation and sarcomere organization are dramatically reduced in smyd1b~(sa15678) single mutant, and almost completely diminish in smyd1a~(mb5); smyd1b~(sa15678) double mutant, but not in smyd1a~(mb5) mutant. Similar defects are also observed in cardiac muscles of smyd1b~(sa15678) mutant. Defective craniofacial and cardiac muscle formation is associated with an upregulation of hsp90 a1 and unc45 b m RNA expression in smyd1b~(sa15678) and smyd1a~(mb5); smyd1b~(sa15678) mutants. Together, our studies indicate that Smyd1 b, but not Smyd1 a, plays a key role in myosin heavy-chain protein expression and sarcomere organization in craniofacial and cardiac muscles. Loss of smyd1 b results in muscle-specific stress response.
关键词	Smyd1 Myosin Sarcomere Craniofacial muscle Cardiac muscle
CNKI专辑号	D;E;A;
CNKI专辑名称	农业科技;医药卫生科技;基础科学;
CNKI专题号	A006;
CNKI专题名称	生物学;
分类号	Q344
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	supported by funding from the U.S. National Institute of Health (NIH) National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR072703 to S. Du) ; supported in part by fellowships from the China Scholarship Council
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/187808
专题	中国科学院海洋研究所
作者单位	1.DepartmentofBiochemistryandMolecularBiology,InstituteofMarineandEnvironmentalTechnology,UniversityofMarylandSchoolofMedicine 2.KeyLaboratoryofExperimentalMarineBiology,CenterforOceanMega-Science,InstituteofOceanology,ChineseAcademyofSciences 3.SchoolofLifeSciences,ShandongUniversityofTechnology
推荐引用方式 GB/T 7714	Shuang Jiao,Rui Xu,Shaojun Du. Smyd1 is essential for myosin expression and sarcomere organization in craniofacial, extraocular, and cardiac muscles. 2021.