Synthesis, characterization, and antioxidant activity of carboxymethyl chitosan derivatives containing sulfonium salt

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	Synthesis, characterization, and antioxidant activity of carboxymethyl chitosan derivatives containing sulfonium salt
	Xueqi SUN ; Jingjing ZHANG ; Yingqi MI ; Qin MIAO ; Wenqiang TAN ; Qing LI ; Zhanyong GUO
发表期刊	Journal of Oceanology and Limnology
ISSN	2096-5508
	2022-01-15
出版年	2022
卷号	v.40 期号:01 页码:287-298
文献类型	CNKI期刊论文
摘要	To improve the solubility and bioactivity of chitosan, a new class of carboxymethyl chitosan derivatives possessing sulfonium salts was successfully designed and synthesized, including Methyl sulfide carboxymethyl chitosan(MCMCS), Ethyl sulfide carboxymethyl chitosan(ECMCS), Propyl sulfide carboxymethyl chitosan(PCMCS), and Butyl sulfide carboxymethyl chitosan(BCMCS). To determine the structure of the new class of the derivatives, methods of the Fourier transform infrared spectroscopy(FT-IR),~1H nuclear magnetic resonance spectrometer( ~1H NMR), and ~(13)C nuclear magnetic resonance spectrometer( ~(13)C NMR) were used. Moreover, the antioxidant activity of the derivatives for three types of free radicals,i.e., hydroxyl radical, superoxide radical, and 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical was evaluated in vitro. In addition, the L929 cells were adopted to test the cytotoxicity of chitosan and its derivatives by CCK-8 assay. The class of the carboxymethyl chitosan derivatives showed a strong scavenging ability against the three free radicals at 1.6 mg/mL, with scavenging rate of over 70% and some up to 100%. At this high rate, the overall cell viability in the toxicity test reached more than 80%, indicating that the synthetic derivative had a little cytotoxicity. The results show that the introduction of carboxymethyl group to chitosan increased the water-solubility of chitosan, and the combination of sulfonate ions with dif ferent chain lengths further enhanced the antioxidant activity of chitosan. Therefore, the sulfonium-containing carboxymethyl chitosan derivatives had excellent bioactivity with good application prospects in food, biomedicine, and medical fields.
关键词	antioxidant activity carboxymethyl chitosan derivative sulfonium salts structural characteristics/synthesis
CNKI专辑号	A;B;
CNKI专辑名称	基础科学;工程科技Ⅰ辑;
CNKI专题号	B014;
CNKI专题名称	化学;
分类号	O636.1
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	Supported by the National Key R&D Program of China (No. 2019YFD0900705) ; the Key Deployment Projects of the Marine Science Research Center of the Chinese Academy of Sciences (No. COMS2020J04) ; the Natural Science Foundation of Shandong Province of China (No. ZR2019BD064)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/187522
专题	中国科学院海洋研究所
作者单位	1.KeyLaboratoryofCoastalBiologyandBioresourceUtilization,YantaiInstituteofCoastalZoneResearch,ChineseAcademyofSciences 2.CenterforOceanMega-Science,ChineseAcademyofSciences 3.UniversityofChineseAcademyofSciences
推荐引用方式 GB/T 7714	Xueqi SUN,Jingjing ZHANG,Yingqi MI,et al. Synthesis, characterization, and antioxidant activity of carboxymethyl chitosan derivatives containing sulfonium salt. 2022.