A ratiometric fluorescent probe based on carbon dots assembly for intracellular lysosomal polarity imaging with wide range response

IOCAS-IR

	A ratiometric fluorescent probe based on carbon dots assembly for intracellular lysosomal polarity imaging with wide range response
	Guangyue Zou ; Shuai Chen ; Nazhen Liu ; Yongliang Yu
发表期刊	Chinese Chemical Letters
ISSN	1001-8417
	2022-02-15
出版年	2022
卷号	v.33 期号:02 页码:233-237
文献类型	CNKI期刊论文
摘要	Lysosomal polarity is considered a key indicator of lysosomal function due to its significant impact on membrane fluidity and enzymatic reactions in lysosomes. Monitoring lysosomal polarity can gain insight into the related physiological and pathological processes and develop new diagnostic methods. However, current fluorescent probes with lysosomal polarity response suffer from narrow linear range, photobleaching and complicated preparation. Herein, a ratiometric fluorescent probe(r-b CDs) for intracellular lysosomal polarity imaging is designed and constructed by amide bond assembly of polarity-sensitive red fluorescent carbon dots(r CDs) and referenced blue fluorescent carbon dots(b CDs). r-b CDs show a much wider linear range of polarity response(orientation polarizability Δf from 0.020 to 0.315) than other probes, and the interference of uneven distribution and instrument factors can be effectively eliminated by ratiometric fluorescent sensing. Imaging of intracellular lysosomal polarity with r-b CDs is implemented to observe the polarity variation caused by the change of cell state and the difference between cancer cells and normal cells. This work provides a promising tool for studying the related physiological and pathological processes and developing new diagnostic methods.
关键词	Carbon dots Polarity Lysosome Ratiometric sensing Wide linear range
CNKI专辑号	B;A;
CNKI专辑名称	工程科技Ⅰ辑;基础科学;
CNKI专题号	A006;B014;
CNKI专题名称	生物学;化学;
分类号	Q503;O657.3
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	financially supported by the National Natural Science Foundation of China (Nos. 21922402, 21874017 and 21727811) ; the Fundamental Research Funds for the Central Universities (No. N2005027)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/187503
专题	中国科学院海洋研究所
作者单位	1.ResearchCenterforAnalyticalSciences,DepartmentofChemistry,CollegeofSciences,NortheasternUniversity 2.KeyLaboratoryofMarineEnvironmentalCorrosionandBio-fouling,InstituteofOceanology,ChineseAcademyofSciences
推荐引用方式 GB/T 7714	Guangyue Zou,Shuai Chen,Nazhen Liu,et al. A ratiometric fluorescent probe based on carbon dots assembly for intracellular lysosomal polarity imaging with wide range response. 2022.