Molecular insights into the circadian clock in marine diatoms

IOCAS-IR

	Molecular insights into the circadian clock in marine diatoms
	Shufeng Zhang ; Yue Wu ; Lin Lin ; Dazhi Wang
发表期刊	Acta Oceanologica Sinica
ISSN	0253-505X
	2022-08-15
出版年	2022
卷号	v.41 期号:08 页码:90-101
文献类型	CNKI期刊论文
摘要	The circadian clock is a fundamental endogenous mechanism of adaptation that coordinates the physiology and behavior of most organisms with diel variations in the external environment to maintain temporal homeostasis.Diatoms are the major primary producers in the ocean. However, little is known about the circadian clock in marine diatoms compared with other organisms. Here, we investigated circadian clock genes, their expression patterns, and responses to environmental stimuli such as light, nitrogen and phosphorus in two marine diatoms,Skeletonema costatum and Phaeodactylum tricornutum, using a combination of q RT-PCR and bioinformatic analysis. We identified 17 and 18 circadian clock genes in P. tricornutum and S. costatum, respectively. Despite significant evolutionary differences, these genes were similar to those of the higher plant Arabidopsis. We also established a molecular model for the marine diatom circadian clock comprising an input pathway, core oscillator, output pathway, and valve effector. Notably, the expression patterns of core clock genes(circadian clock associated 1(CCA1), late elongated hypocotyl(LHY) and timing of cab 1(TOC1)) in both species differed from those of terrestrial plants. Furthermore, the expression of these genes was influenced by variations in ambient light, nitrogen and phosphorus availability. Although marine diatoms and higher plants share common circadian clock components, their clock genes have diverged throughout evolution, likely as a result of adapting to contrasting environments.
关键词	circadian clock circadian genes marine diatoms Phaeodactylum tricornutum Skeletonema costatum
CNKI专辑号	A;
CNKI专辑名称	基础科学;
CNKI专题号	A006;
CNKI专题名称	生物学;
分类号	Q943.2
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	The National Natural Science Foundation of China under contract Nos 41425021 and 41706131 ; the National Key Research and Development Program of China under contract No. 2017YFC1404302 ; the “Ten Thousand Talents Program” for Leading Talents in Science and Technological Innovation to Dazhi Wang
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/187349
专题	中国科学院海洋研究所
作者单位	1.StateKeyLaboratoryofMarineEnvironmentalScience,XiamenUniversity 2.CollegeoftheEnvironmentandEcology,XiamenUniversity 3.KeyLaboratoryofMarineEcology&EnvironmentalSciences,InstituteofOceanology,ChineseAcademyofSciences
推荐引用方式 GB/T 7714	Shufeng Zhang,Yue Wu,Lin Lin,et al. Molecular insights into the circadian clock in marine diatoms. 2022.