Variations in nano- and pico-eukaryotic phytoplankton assemblages in the Qinhuangdao green-tide area

IOCAS-IR

	Variations in nano- and pico-eukaryotic phytoplankton assemblages in the Qinhuangdao green-tide area
	Weiqian ZHANG ; Hongbin HAN ; Limei QIU ; Chao LIU ; Qingchun ZHANG ; Guizhong ZHOU
发表期刊	Journal of Oceanology and Limnology
ISSN	2096-5508
	2022-11-15
出版年	2022
卷号	v.40 期号:06 页码:368-383
文献类型	CNKI期刊论文
摘要	Qinhuangdao coastal waters have been frequently hitting by nano-and pico-eukaryotic phytoplankton(NPEP) blooms and green tides(macroalgal blooms) in the recent decade. However,understanding about the impacts of environmental factors and the green tides on the NPEP assemblages in this area is limited. In this study, the composition of NPEP assemblages and their variations were analyzed via amplicon sequence variants(ASVs) assay based on amplicon high-throughput sequencing data with the 18S V4 region as a targeted gene in the Qinhuangdao green-tide area during the green tide. Consequently, average NPEP ef fective sequences and ASVs of 178 000 and 200 were obtained from each sample, respectively.Although there were 25 classes, 110 genera, and 97 species of NPEP were identified and annotated, the proportions of annotated ASVs at genus and species levels were only 44.7% and 17.8%, respectively. The NPEP communities had a seasonal succession from diatom-dominated to dinofl agellate-dominated. During the three investigations, Skeletonema, Karlodinium, and Gonyaulax were the most dominant genera in May,August, and September, respectively. Species diversity and the abundance of NPEP communities could be increased by a high content of dissolved organic nitrogen(DON) and dissolved organic phosphorus(DOP) but inhibited by low dissolved inorganic phosphorus content. The outbreak of green tides could alter the composition and content of nutrients and accelerate the succession of the NPEP communities from diatom-dominated to dinofl agellate-dominated under the background of a seasonal increase in seawater temperature. These results preliminarily revealed the impacts of the recurrent occurrences of green tides on the NPEP assemblages in the Qinhuangdao green-tide area exhibiting high DON content and dissolved inorganic nitrogen/phosphorus ratio.
关键词	nano- and pico-eukaryotic phytoplankton high-throughput sequencing green tide eutrophication Qinhuangdao
CNKI专辑号	A;B;
CNKI专辑名称	基础科学;工程科技Ⅰ辑;
CNKI专题号	B027;
CNKI专题名称	环境科学与资源利用;
分类号	X52
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	Supported by the National Key R&D Program of China (No. 2019YFC14079000) ; the National S&T Basic Resources Investigation Program of China (No. 2018FY100206) from the Ministry of Science and Technology (MoST)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/187256
专题	中国科学院海洋研究所
作者单位	1.QingdaoUniversityofScienceandTechnology 2.CASandShandongProvinceKeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,CASCenterforOceanMega-Science,ChineseAcademyofSciences 3.LaboratoryforMarineFisheriesScienceandFoodProductionProcesses,PilotNationalLaboratoryforMarineScienceandTechnology(Qingdao) 4.KeyLaboratoryofMarineEco-EnvironmentalScienceandTechnology,FirstInstituteofOceanography,MinistryofNaturalResources 5.CenterofOceanMegaScience,ChineseAcademyofSciences 6.CASKeyLaboratoryofMarineEcologyandEnvironmentalSciences,InstituteofOceanology,ChineseAcademyofSciences 7.LaboratoryforMarineEcologyandEnvironmentalScience,PilotNationalLaboratoryforMarineScienceandTechnology(Qingdao) 8.UniversityofChineseAcademyofSciences
推荐引用方式 GB/T 7714	Weiqian ZHANG,Hongbin HAN,Limei QIU,et al. Variations in nano- and pico-eukaryotic phytoplankton assemblages in the Qinhuangdao green-tide area. 2022.