Screening for lipophilic marine toxins and their potential producers in coastal waters of Weihai in autumn, 2020

IOCAS-IR

	Screening for lipophilic marine toxins and their potential producers in coastal waters of Weihai in autumn, 2020
	Huixia GENG; Hongxiao SUN; Chao LIU ; Fanzhou KONG; Qingchun ZHANG ; Tian YAN ; Rencheng YU
发表期刊	Journal of Oceanology and Limnology
ISSN	2096-5508
	2022-11-15
出版年	2022
卷号	v.40 期号:06 页码:140-152
文献类型	CNKI期刊论文
摘要	Lipophilic marine toxins(LMTs) produced by some microalgae in the sea could accumulate in shellfish and pose potential threats to the health of seafood consumers. Phytoplankton and shellfish samples were collected from coastal waters of Weihai in Shandong Peninsula, China in autumn, 2020, and screened for lipophilic marine toxins and their potential producers using liquid chromatography-tandem mass spectrometry(LC-MS/MS) analysis and high throughput sequencing of partial DNA(V4 region of the 18S rRNA gene) extracted from phytoplankton. Pectenotoxin-2(PTX2), trace amounts of azaspiracid(AZA1 or AZA40), and 13-desmethyl spirolide C(13-DesMe-C) were detected in phytoplankton samples, while PTX2and gymnodimine(GYM) were detected in shellfish samples. The toxin content in shellfish samples was much lower than the regulatory limit or values reported previously. Results suggest that lipophilic marine toxins should have low risk in coastal waters of Weihai in autumn. Based on the data of high throughput sequencing, the OTUs were assigned to 5 identified species of Alexandrium, including A. ostenfeldii capable of producing 13-DesMe-C and GYM. Two OTUs were found closely related to the toxic species in genus Dinophysis, but it is impossible to assign them to any identified species due to the low resolving power of the V4 region for Dinophysis. The OTUs could not be assigned to any identified species in the genus Azadinium,suggesting the existence of unidentified species in this region.
关键词	harmful algal blooms phytoplankton shellfish lipophilic marine toxins (LMTs) Yellow Sea liquid chromatography-tandem mass spectrometry
CNKI专辑号	A;B;
CNKI专辑名称	基础科学;工程科技Ⅰ辑;
CNKI专题号	A010;B027;
CNKI专题名称	海洋学;环境科学与资源利用;
分类号	X55
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	Supported by the Pilot National Laboratory for Marine Science and Technology (No. 2021QNLM040001-2) ; the Science and Technology Basic Resources Investigation Program (No. 2018FY100200) ; the National Key R&D Program (No. 2017YFC1600701) ; the CAS-CSIRO BAU project of the Chinese Academy of Sciences (No. GJHZ201973) ; the National Natural Science Foundation of China (No. 42106206)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/187255
专题	中国科学院海洋研究所
作者单位	1.CASKeyLaboratoryofMarineEcologyandEnvironmentalSciences,InstituteofOceanology,ChineseAcademyofSciences 2.CenterforOceanMega-Science,ChineseAcademyofSciences 3.UniversityofChineseAcademyofSciences 4.LaboratoryforMarineEcologyandEnvironmentalScience,PilotNationalLaboratoryforMarineScienceandTechnology(Qingdao)
推荐引用方式 GB/T 7714	Huixia GENG,Hongxiao SUN,Chao LIU,et al. Screening for lipophilic marine toxins and their potential producers in coastal waters of Weihai in autumn, 2020. 2022.