Integrated transcriptomic and metabolomic approaches reveal molecular response and potential biomarkers of the deep-sea mussel <i>Gigantidas</i> <i>platifrons </i>to copper exposure

doi:10.1016/j.jhazmat.2024.134612

	*Integrated transcriptomic and metabolomic approaches reveal molecular response and potential biomarkers of the deep-sea mussel Gigantidas* platifrons to copper exposure**
	He, Yameng 1; Zhou, Li 1; Wang, Minxiao 1; Zhong, Zhaoshan1 ; Chen, Hao1 ; Lian, Chao 1; Zhang, Huan 1; Wang, Hao 1; Cao, Lei1 ; Li, Chaolun1,2,3,4,5
	2024-07-15
发表期刊	JOURNAL OF HAZARDOUS MATERIALS
ISSN	0304-3894
卷号	473 页码:13
通讯作者	Zhou, Li([email protected]) ; Li, Chaolun([email protected])
摘要	Metal pollution caused by deep-sea mining activities has potential detrimental effects on deep-sea ecosystems. However, our knowledge of how deep-sea organisms respond to this pollution is limited, given the challenges of remoteness and technology. To address this, we conducted a toxicity experiment by using deep-sea mussel Gigantidas platifrons as model animals and exposing them to different copper (Cu) concentrations (50 and 500 mu g/ L) for 7 days. Transcriptomics and LC-MS-based metabolomics methods were employed to characterize the profiles of transcription and metabolism in deep-sea mussels exposed to Cu. Transcriptomic results suggested that Cu toxicity significantly affected the immune response, apoptosis, and signaling processes in G. platifrons. Metabolomic results demonstrated that Cu exposure disrupted its carbohydrate metabolism, anaerobic meta- bolism and amino acid metabolism. By integrating both sets of results, transcriptomic and metabolomic, we find that Cu exposure significantly disrupts the metabolic pathway of protein digestion and absorption in G. platifrons . Furthermore, several key genes (e.g., heat shock protein 70 and baculoviral IAP repeat -containing protein 2/3) and metabolites (e.g., alanine and succinate) were identified as potential molecular biomarkers for deep-sea mussel 's responses to Cu toxicity. This study contributes novel insight for assessing the potential effects of deep-sea mining activities on deep-sea organisms.
关键词	Mytilidae Deep-sea Metals Multi-omics Toxic effects Biomarkers
DOI	10.1016/j.jhazmat.2024.134612
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2022YFC2804003]; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42020401]; National Natural Science Foundation of China[42276153]; National Natural Science Foundation of China[42030407]; Key Research Program of Frontier Sciences, CAS[ZDBS-LY-DQC032]; State Key Laboratory of Microcbial Technology Open Projects Fund[M2023-10]
WOS研究方向	Engineering ; Environmental Sciences & Ecology
WOS类目	Engineering, Environmental ; Environmental Sciences
WOS记录号	WOS:001243332700001
出版者	ELSEVIER
WOS关键词	BATHYMODIOLUS-AZORICUS ; RUDITAPES-PHILIPPINARUM ; ANAEROBIC METABOLISM ; METAL CONTAMINATION ; GENE-EXPRESSION ; CADMIUM ; STRESS ; SIGNAL ; INVERTEBRATES ; MECHANISMS
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186597
专题	海洋生态与环境科学重点实验室
通讯作者	Zhou, Li; Li, Chaolun
作者单位	1.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou 510301, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 5.Laoshan Lab, Qingdao 266237, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	He, Yameng,Zhou, Li,Wang, Minxiao,et al. Integrated transcriptomic and metabolomic approaches reveal molecular response and potential biomarkers of the deep-sea mussel Gigantidas platifrons to copper exposure[J]. JOURNAL OF HAZARDOUS MATERIALS,2024,473:13.
APA	He, Yameng.,Zhou, Li.,Wang, Minxiao.,Zhong, Zhaoshan.,Chen, Hao.,...&Li, Chaolun.(2024).Integrated transcriptomic and metabolomic approaches reveal molecular response and potential biomarkers of the deep-sea mussel Gigantidas platifrons to copper exposure.JOURNAL OF HAZARDOUS MATERIALS,473,13.
MLA	He, Yameng,et al."Integrated transcriptomic and metabolomic approaches reveal molecular response and potential biomarkers of the deep-sea mussel Gigantidas platifrons to copper exposure".JOURNAL OF HAZARDOUS MATERIALS 473(2024):13.