Development of DNA Insertion-specific Markers Based on the Intergenic Region of <i>Oplegnathus punctatus Cdkn1</i>/<i>srsf3</i> for Sex Identification

doi:10.1007/s10126-024-10336-6

IOCAS-IR > 实验海洋生物学重点实验室

	*Development of DNA Insertion-specific Markers Based on the Intergenic Region of Oplegnathus punctatus Cdkn1/srsf3* for Sex Identification**
	Ma, Yuting 1,2,3; Xiao, Yongshuang 1,2,3; Xiao, Zhizhong 1,2,3; Li, Jun 1,2,3
	2024-06-14
发表期刊	MARINE BIOTECHNOLOGY
ISSN	1436-2228
页码	9
通讯作者	Xiao, Yongshuang([email protected]) ; Li, Jun([email protected])
摘要	Spotted knifejaw (Oplegnathus punctatus) is a marine economic fish with high food and ecological value, and its growth process has obvious male and female sexual dimorphism, with males growing significantly faster than females. However, the current sex identification technology is not yet mature, which will limit the growth rate of O. punctatus aquaculture and the efficiency of separate sex breeding, so the development of efficient sex molecular markers is imperative. This study identified a 926 bp DNA insertion fragment in the cdkn1/srsf3 intergenic region of O. punctatus males through whole-genome scanning, comparative genomics, and structural variant analysis. A pair of primers was designed based on the insertion information of the Y chromosome intergenic region in male individuals. Agarose gel electrophoresis revealed the amplification of two DNA fragments, 1118 bp and 192 bp, in male O. punctatus individuals. The 926 bp fragment was identified as the insertion in the intergenic region of cdkn1/srsf3 in males, while only a single 192 bp DNA fragment was amplified in females. The biological sex of the individuals identified in this manner was consistent with their known phenotypic sex. In this study, we developed a method to detect DNA insertion variants in the intergenic region of O. punctatus. Additionally, we introduced a new DNA marker for the rapid identification of the sex of O. punctatus, which enhances detection efficiency. The text has important reference significance and application value in sex identification, all-male breeding, and lineage selection. It provides new insights into the regulation of variation in the intergenic region of cdkn1/srsf3 genes and the study of RNA shearing.
关键词	Oplegnathus punctatus Sex molecular marker Genetic sex identification cdkn1/srsf3 Intergenic mutation
DOI	10.1007/s10126-024-10336-6
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China; Chinese Academy of Sciences
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS类目	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号	WOS:001248184500001
出版者	SPRINGER
WOS关键词	SPOTTED KNIFEJAW ; SR PROTEINS ; EXPRESSION ; FEMALE ; GENE ; TRANSCRIPTION ; ALIGNMENT ; SEQUENCE ; P21
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186588
专题	实验海洋生物学重点实验室
通讯作者	Xiao, Yongshuang; Li, Jun
作者单位	1.Chinese Acad Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Ma, Yuting,Xiao, Yongshuang,Xiao, Zhizhong,et al. Development of DNA Insertion-specific Markers Based on the Intergenic Region of Oplegnathus punctatus Cdkn1/srsf3 for Sex Identification[J]. MARINE BIOTECHNOLOGY,2024:9.
APA	Ma, Yuting,Xiao, Yongshuang,Xiao, Zhizhong,&Li, Jun.(2024).Development of DNA Insertion-specific Markers Based on the Intergenic Region of Oplegnathus punctatus Cdkn1/srsf3 for Sex Identification.MARINE BIOTECHNOLOGY,9.
MLA	Ma, Yuting,et al."Development of DNA Insertion-specific Markers Based on the Intergenic Region of Oplegnathus punctatus Cdkn1/srsf3 for Sex Identification".MARINE BIOTECHNOLOGY (2024):9.