Molecular Characterization of the Actin Gene and 5′ Flanking Sequence from Brown Macroalga <i>Saccharina japonica</i> (Laminariales, Phaeophyta)

doi:10.3390/jmse12060887

IOCAS-IR > 实验海洋生物学重点实验室

	*Molecular Characterization of the Actin Gene and 5′ Flanking Sequence from Brown Macroalga Saccharina japonica* (Laminariales, Phaeophyta)**
	Xu, Hao 1,2,3,4; Wang, Zhenghua 1,2,3,4; Zhang, Yichen 1,2,5; Jiang, Peng 1,2,3
	2024-06-01
发表期刊	JOURNAL OF MARINE SCIENCE AND ENGINEERING
卷号	12 期号:6 页码:13
通讯作者	Jiang, Peng([email protected])
摘要	The brown macroalga Saccharina japonica (Laminariales, Phaeophyta) is the most productive cultured seaweed in the world. In order to improve the biosafety of transgenic kelp, it is necessary to develop endogenous constitutive promoters, replacing those of virus origin. In this study, the housekeeping actin gene from S. japonica (SjACT) was found to contain three exons and two introns, representing a unique actin gene structure pattern in brown algae. Additionally, the 5 ' upstream region was obtained using genome walking, and fused to the reporter gene lacZ or EGFP to construct promoter-detective vectors. Using an established genetic transformation system, kelps in different life-cycle stages were transformed. The detection results showed that, in the diploid sporophyte stage, the transient expression from the lacZ gene could be observed in the frond, stipe, or holdfast of kelps, indicating a manner of being non-tissue-specific. And, in the haploid gametophyte stage of S. japonica, the fluorescence of the expressed EGFP were detected in vivo in gametophyte cells of both genders. These results indicate that the promoter of the SjACT gene (pSjACT) functions in a constitutive manner and is expected to be a key endogenous element in the genetic manipulation of kelps.
关键词	actin Biolistic GFP lacZ constitutive promoter Saccharina japonica transient expression
DOI	10.3390/jmse12060887
收录类别	SCI
语种	英语
资助项目	Natural Science Foundation of Qingdao City[23-2-1-172-zyyd-jch]; Science & Technology Basic Resources Investigation Program of China[2018FY100205]; Key R&D Program of Shandong Province[2019GSF107012]; National Key R&D Program of China[2018YFD0901500]; Promotive Research Fund for Excellent Young and Middle-aged Scientists of Shandong Province[2010BSB02009]
WOS研究方向	Engineering ; Oceanography
WOS类目	Engineering, Marine ; Engineering, Ocean ; Oceanography
WOS记录号	WOS:001257569700001
出版者	MDPI
WOS关键词	STRUCTURAL FEATURES ; EXPRESSION ; PHYLOGENY ; PROMOTER ; ALGAE
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186381
专题	实验海洋生物学重点实验室
通讯作者	Jiang, Peng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Tianjin Normal Univ, Coll Life Sci, Tianjin 300387, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Xu, Hao,Wang, Zhenghua,Zhang, Yichen,等. Molecular Characterization of the Actin Gene and 5′ Flanking Sequence from Brown Macroalga Saccharina japonica (Laminariales, Phaeophyta)[J]. JOURNAL OF MARINE SCIENCE AND ENGINEERING,2024,12(6):13.
APA	Xu, Hao,Wang, Zhenghua,Zhang, Yichen,&Jiang, Peng.(2024).Molecular Characterization of the Actin Gene and 5′ Flanking Sequence from Brown Macroalga Saccharina japonica (Laminariales, Phaeophyta).JOURNAL OF MARINE SCIENCE AND ENGINEERING,12(6),13.
MLA	Xu, Hao,et al."Molecular Characterization of the Actin Gene and 5′ Flanking Sequence from Brown Macroalga Saccharina japonica (Laminariales, Phaeophyta)".JOURNAL OF MARINE SCIENCE AND ENGINEERING 12.6(2024):13.