MAPK/ERK-PK(Ser11) pathway regulates divergent thermal metabolism of two congeneric oyster species

doi:10.1016/j.isci.2024.110321

	MAPK/ERK-PK(Ser11) pathway regulates divergent thermal metabolism of two congeneric oyster species
	Wang, Chaogang1,2,3,4 ; Du, Mingyang 1,2,3,4; Jiang, Zhuxiang 1,2,3,4; Cong, Rihao 1,2,3,7,8,9; Wang, Wei 1,2,6,7,8,9; Zhang, Taiping 1,2,3,4; Chen, Jincheng 1,2,3,4; Zhang, Guofan 1,2,3,5,7,8,9; Li, Li 1,2,4,5,6,7,9
	2024-07-19
发表期刊	ISCIENCE
卷号	27 期号:7 页码:22
通讯作者	Li, Li([email protected])
摘要	Pyruvate kinase (PK), as a key rate-limiting enzyme in glycolysis, has been widely used to assess the stress tolerance and sensitivity of organisms. However, its phosphorylation regulatory mechanisms mainly focused on human cancer research, with no reports in marine organisms. In this study, we firstly reported a conserved PK Ser11 phosphorylation site in mollusks, which enhanced enzyme activity by promoting substrate binding, thereby regulating divergent thermal metabolism of two allopatric congeneric oyster species with differential habitat temperature. It was phosphorylated by ERK kinase, and regulated by the classical MAPK pathway. The MAPK/ERK-PK signaling cascade responded to increased environmental temperature and exhibited stronger activation pattern in the relatively thermotolerant species ( Crassos- trea angulata ), indicating its involvement in shaping temperature adaptation. These findings highlight the presence of complex and unique phosphorylation-mediated signaling transduction mechanisms in marine organisms, and provide new insights into the evolution and function of the crosstalk between classical pathways.
DOI	10.1016/j.isci.2024.110321
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2022YFD2400304]; Key Research and Development Program of Shandong[2022LZGC015]; Key Research and Development Program of Shandong[ZFJH202309]; National Natural Science Foundation of China[32101353]; Young Elite Scientists Sponsorship Program by China Association of Science and Technology[2021QNRC001]; China Agriculture Research System of MOF and MARA[CARS-49]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:001266278500001
出版者	CELL PRESS
WOS关键词	MYTILUS-GALLOPROVINCIALIS ; PYRUVATE-KINASE ; HEAT-STRESS ; PROTEIN EXPRESSION ; OXIDATIVE STRESS ; PHOSPHORYLATION ; RESPONSES ; PKM2 ; CARBOHYDRATE ; ACTIVATION
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186321
专题	海洋生态与环境科学重点实验室
通讯作者	Li, Li
作者单位	1.Chinese Acad Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan, Peoples R China 6.Qingdao Marine Sci & Technol Ctr, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Shandong, Peoples R China 7.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao, Peoples R China 8.Shandong Technol Innovat Ctr Oyster Seed Ind, Qingdao, Peoples R China 9.Southern Marine Sci & Engn Guangdong Lab Zhanjiang, Zhanjiang, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Chaogang,Du, Mingyang,Jiang, Zhuxiang,et al. MAPK/ERK-PK(Ser11) pathway regulates divergent thermal metabolism of two congeneric oyster species[J]. ISCIENCE,2024,27(7):22.
APA	Wang, Chaogang.,Du, Mingyang.,Jiang, Zhuxiang.,Cong, Rihao.,Wang, Wei.,...&Li, Li.(2024).MAPK/ERK-PK(Ser11) pathway regulates divergent thermal metabolism of two congeneric oyster species.ISCIENCE,27(7),22.
MLA	Wang, Chaogang,et al."MAPK/ERK-PK(Ser11) pathway regulates divergent thermal metabolism of two congeneric oyster species".ISCIENCE 27.7(2024):22.