The small GTPase Cdc42 regulates shell field morphogenesis in a gastropod mollusk

doi:10.1016/j.ydbio.2024.06.012

IOCAS-IR > 实验海洋生物学重点实验室

	The small GTPase Cdc42 regulates shell field morphogenesis in a gastropod mollusk
	Liu, Xinyu 1,4; Huan, Pin1,2,3,4 ; Liu, Baozhong1,3
	2024-11-01
发表期刊	DEVELOPMENTAL BIOLOGY
ISSN	0012-1606
卷号	515 页码:7-17
通讯作者	Huan, Pin([email protected])
摘要	In most mollusks (conchiferans), the early tissue responsible for shell development, namely, the shell field, shows a common process of invagination during morphogenesis. Moreover, lines of evidence indicated that shell field invagination is not an independent event, but an integrated output reflecting the overall state of shell field morphogenesis. Nevertheless, the underlying mechanisms of this conserved process remain largely unknown. We previously found that actomyosin networks (regularly organized filamentous actin (F-actin) and myosin) may play essential roles in this process by revealing the evident aggregation of F-actin in the invaginated region and demonstrating that nonmuscle myosin II (NM II) is required for invagination in the gastropod Lottia peitaihoensis ( = Lottia goshimai ). Here, we investigated the roles of the Rho family of small GTPases (RhoA, Rac1, and Cdc42) to explore the upstream regulators of actomyosin networks. Functional assays using small molecule inhibitors suggested that Cdc42 modulates key events of shell field morphogenesis, including invagination and cell rearrangements, while the roles of RhoA and Rac1 may be nonspecific or negligible. Further investigations revealed that the Cdc42 protein was concentrated on the apical side of shell field cells and colocalized with F-actin aggregation. The aggregation of these two molecules could be prevented by treatment with Cdc42 inhibitors. These findings suggest a possible regulatory cascade of shell field morphogenesis in which Cdc42 recruits F-actin (actomyosin networks) on the apical side of shell field cells, which then generates resultant mechanical forces that mediate correct shell field morphogenesis (cell shape changes, invagination and cell rearrangement). Our results emphasize the roles of the cytoskeleton in early shell development and provide new insights into molluscan shell evolution.
关键词	Lottia peitaihoensis Shell development Cdc42 F-actin Shell field morphogenesis
DOI	10.1016/j.ydbio.2024.06.012
收录类别	SCI
语种	英语
资助项目	Science & Technology Innovation Project of Laoshan Laboratory[LSKJ202203002]; National Natural Science Foundation of China[41776157]; National Natural Science Foundation of China[42076123]; Earmarked fund for CARS-49; Taishan Scholar Project Fund of Shandong Province of China[tsqn202103129]
WOS研究方向	Developmental Biology
WOS类目	Developmental Biology
WOS记录号	WOS:001266276600001
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS关键词	EMBRYONIC-DEVELOPMENT ; RHO-GTPASES ; CO-OPTION ; PERIOSTRACUM ; EXPRESSION ; ARCHITECTURE ; VENERIDAE ; FILOPODIA ; EVOLUTION ; PATHWAY
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186312
专题	实验海洋生物学重点实验室
通讯作者	Huan, Pin
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liu, Xinyu,Huan, Pin,Liu, Baozhong. The small GTPase Cdc42 regulates shell field morphogenesis in a gastropod mollusk[J]. DEVELOPMENTAL BIOLOGY,2024,515:7-17.
APA	Liu, Xinyu,Huan, Pin,&Liu, Baozhong.(2024).The small GTPase Cdc42 regulates shell field morphogenesis in a gastropod mollusk.DEVELOPMENTAL BIOLOGY,515,7-17.
MLA	Liu, Xinyu,et al."The small GTPase Cdc42 regulates shell field morphogenesis in a gastropod mollusk".DEVELOPMENTAL BIOLOGY 515(2024):7-17.