Comparative transcriptomic analysis of ovary and testis reveals sex-related genes in roughskin sculpin <i>Trachidermus fasciatus</i>

doi:10.1007/s00343-024-3285-8

	Comparative transcriptomic analysis of ovary and testis reveals sex-related genes in roughskin sculpin Trachidermus fasciatus
	Xue, Dongxiu1,2 ; Xia, Sudong 3; Xing, Tengfei 1,2; Gao, Yuqian 3; Liu, Jinxian 1,2
	2024-07-06
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
页码	10
通讯作者	Xue, Dongxiu([email protected])
摘要	The mechanisms underlying sex determination and differentiation have long intrigued researchers in the fields of development and evolutionary biology. The roughskin sculpin (Trachidermus fasciatus Heckel), displaying sexual dimorphism, provides an ideal model for studying the mechanisms. However, both genetic and genomic information concerning sex determination and differentiation, such as gonadal transcriptome data in roughskin sculpin, are lacking. Here, we present the first gonadal transcriptomes of roughskin sculpin and identify sex-related genes. We identified 8 531 differentially expressed genes (DEGs), among them 4 065 were upregulated in the ovary and 4 466 upregulated in the testis. Several sex-related gene ontology (GO) terms were enriched in ovary-biased genes, including "binding of sperm to zona pellucida", "egg coat formation", "positive regulation of acrosome reaction", "cell division", and "cell cycle", while the GO terms such as "spermatogenesis", "sperm axoneme assembly", "cilium assembly", "cilium movement", and "cilium movement involved in cell motility" were enriched in testis-biased genes. Moreover, six KEGG pathways were significantly enriched in the ovary, whereas only one was enriched in the testis. Of these DEGs, 40 sex-related genes were identified, which including 26 testis-biased genes (such as Dmrtb1, Gsdf, Sox9b, Wnt4b, Tcp11l2, and Efhb), and 14 ovary-biased genes (such as Cyp19a1a, Foxh1, Foxr1, Gdf3, Hsd17b12, and Igf2bp3). This gonadal transcript dataset would broaden our understanding of sex determination and differentiation mechanisms in roughskin sculpin, expand the genomic database, support future studies on sex-related gene functions, and facilitate molecular biology research into roughskin sculpin.
关键词	Trachidermus fasciatus transcriptome ovary testis sex-related genes
DOI	10.1007/s00343-024-3285-8
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31972793]; National Natural Science Foundation of China[31502169]; Key Scientific Research Project in Universities and Colleges in Tianjin[2022ZD004]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:001263497800001
出版者	SCIENCE PRESS
WOS关键词	WNT4 EXPRESSION ; DIFFERENTIATION ; HISAT ; SOX9
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186291
专题	海洋生态与环境科学重点实验室
通讯作者	Xue, Dongxiu
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China 3.Dept Fishery Sci, Tianjin Key Lab Aqua Ecol & Aquaculture, Tianjin 300380, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Xue, Dongxiu,Xia, Sudong,Xing, Tengfei,et al. Comparative transcriptomic analysis of ovary and testis reveals sex-related genes in roughskin sculpin Trachidermus fasciatus[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2024:10.
APA	Xue, Dongxiu,Xia, Sudong,Xing, Tengfei,Gao, Yuqian,&Liu, Jinxian.(2024).Comparative transcriptomic analysis of ovary and testis reveals sex-related genes in roughskin sculpin Trachidermus fasciatus.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,10.
MLA	Xue, Dongxiu,et al."Comparative transcriptomic analysis of ovary and testis reveals sex-related genes in roughskin sculpin Trachidermus fasciatus".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2024):10.