Comparative assessment of the intragenomic variations of dinoflagellate <i>Tripos</i> species through single-cell sequencing

doi:10.1016/j.marpolbul.2024.116690

	*Comparative assessment of the intragenomic variations of dinoflagellate Tripos* species through single-cell sequencing**
	Huang, Xianliang 1,2,3,4; Li, Yingchao 1,2,3,4; Du, Haina 1,2,3,4; Chen, Nansheng 1
	2024-09-01
发表期刊	MARINE POLLUTION BULLETIN
ISSN	0025-326X
卷号	206 页码:12
通讯作者	Chen, Nansheng([email protected])
摘要	Tripos is a large dinoflagellate genus widely distributed in the world 's oceans. Morphology-based species identification is inconclusive due to high morphological intraspecific variability. Metabarcoding analysis has been demonstrated to be effective for species identification and tracking their spatiotemporal dynamics. However, accumulating evidence suggests high levels of intragenomic variations (IGVs) are common in many algae, leading to concerns about overinterpretation of molecular diversity in metabarcoding studies. In this project, we evaluated and compared IGVs in Tripos species by conducting the first high-throughput sequencing (HTS) of 18S rDNA V4 of Tripos single cells. High numbers of haplotypes (19 -172) were identified in each of the 30 Tripos cells. Each cell contained one dominant haplotype with high relative abundance and many haplotypes with lower abundances. Thus, the presence of multiple minor haplotypes substantially overestimate the molecular diversity identified in metabarcoding analysis, which encompass not only interspecific and intraspecific diversities, but high levels of IGVs.
关键词	Tripos Single cell analysis Intra-genomic variations (IGVs) Molecular diversity Metabarcoding
DOI	10.1016/j.marpolbul.2024.116690
收录类别	SCI
语种	英语
资助项目	Marine S&T Fund of Shandong Province for Pilot Qingdao National Laboratory for Marine Science and Technology[LSKJ202203700]; National Key Research and Development Program of China[2022YFC3105200]; Taishan Scholar Project Special Fund; Natural Science Foundation of China[42176162]; Strategic Priority Research Program of Chinese Academy of Sciences[XDB42000000]; Chinese Academy of Sciences Pioneer Hundred Talents Program
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS类目	Environmental Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:001273705900001
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS关键词	INTERNAL TRANSCRIBED SPACER ; HARMFUL ALGAL BLOOMS ; RED-TIDE ; MOLECULAR DIVERSITY ; PROTIST DIVERSITY ; RARE BIOSPHERE ; CERATIUM FURCA ; RIBOSOMAL DNA ; GENUS TRIPOS ; RDNA OPERON
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186276
专题	海洋生态与环境科学重点实验室
通讯作者	Chen, Nansheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China 3.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing 100039, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Huang, Xianliang,Li, Yingchao,Du, Haina,et al. Comparative assessment of the intragenomic variations of dinoflagellate Tripos species through single-cell sequencing[J]. MARINE POLLUTION BULLETIN,2024,206:12.
APA	Huang, Xianliang,Li, Yingchao,Du, Haina,&Chen, Nansheng.(2024).Comparative assessment of the intragenomic variations of dinoflagellate Tripos species through single-cell sequencing.MARINE POLLUTION BULLETIN,206,12.
MLA	Huang, Xianliang,et al."Comparative assessment of the intragenomic variations of dinoflagellate Tripos species through single-cell sequencing".MARINE POLLUTION BULLETIN 206(2024):12.