Investigating the molecular mechanisms of Pseudalteromonas sp. LD-B1 ' s algicidal effects on the harmful alga Heterosigma akashiwo

doi:10.1016/j.ecoenv.2024.116690

	Investigating the molecular mechanisms of Pseudalteromonas sp. LD-B1 ' s algicidal effects on the harmful alga Heterosigma akashiwo
	Xu, Mingyang 1,2; Chen, Yujiao 1,2; Chen, Lei1,2 ; Chen, Yifan 1,2; Yin, Xueyao 1,2; Ji, Nanjing 1,2,3,4; Cai, Yuefeng 1,2; Sun, Song4 ; Shen, Xin 1,2
	2024-09-01
发表期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN	0147-6513
卷号	282 页码:10
通讯作者	Ji, Nanjing([email protected]) ; Shen, Xin([email protected])
摘要	Heterosigma akashiwo is a harmful algal bloom species that causes significant detrimental effects on marine ecosystems worldwide. The algicidal bacterium Pseudalteromonas sp. LD-B1 has demonstrated potential effectiveness in mitigating these blooms. However, the molecular mechanisms underlying LD-B1's inhibitory effects on H. akashiwo remain poorly understood. In this study, we employed the comprehensive methodology, including morphological observation, assessment of photosynthetic efficiency (Fv/Fm), and transcriptomic analysis, to investigate the response of H. akashiwo to LD-B1. Exposure to LD-B1 resulted in a rapid decline of H. akashiwo's Fv/Fm ratio, with cells transitioning to a rounded shape within 2 hours, subsequently undergoing structural collapse and cytoplasmic leakage. Transcriptomic data revealed sustained downregulation of photosynthetic genes, indicating impaired functionality of the photosynthetic system. Additionally, genes related to the respiratory electron transfer chain and antioxidant defenses were consistently downregulated, suggesting prolonged oxidative stress beyond the cellular antioxidative capacity. Notably, upregulation of autophagyrelated genes was observed, indicating autophagic responses in the algal cells. This study elucidates the molecular basis of LD-B1's algicidal effects on H. akashiwo, advancing our understanding of algicidal mechanisms and contributing to the development of effective strategies for controlling harmful algal blooms.
关键词	Pseudalteromonas sp. LD-B1 Heterosigma akashiwo Algicidal effects Transcriptomic analysis
DOI	10.1016/j.ecoenv.2024.116690
收录类别	SCI
语种	英语
资助项目	Science and Technology Innovation Fund (JASTIF)[CX (22) 2032]; Natural Science Foundation of China[42006135]; Lianyungang Postdoctoral Science Foundation[ZKK2021021]; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
WOS类目	Environmental Sciences ; Toxicology
WOS记录号	WOS:001268381900001
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS关键词	OXIDATIVE STRESS ; ELECTRON-TRANSPORT ; CELL-DEATH ; GENERATION ; MACHINERY ; AUTOPHAGY ; CHAIN ; GENE
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186216
专题	海洋生态与环境科学重点实验室
通讯作者	Ji, Nanjing; Shen, Xin
作者单位	1.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Bioresources & Environm, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China 2.Jiangsu Ocean Univ, Coinnovat Ctr Jiangsu Marine Bioind Technol, Lianyungang 222005, Peoples R China 3.Jiangsu Marine Resources Dev Res Inst, Lianyungang 222005, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Xu, Mingyang,Chen, Yujiao,Chen, Lei,et al. Investigating the molecular mechanisms of Pseudalteromonas sp. LD-B1 ' s algicidal effects on the harmful alga Heterosigma akashiwo[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2024,282:10.
APA	Xu, Mingyang.,Chen, Yujiao.,Chen, Lei.,Chen, Yifan.,Yin, Xueyao.,...&Shen, Xin.(2024).Investigating the molecular mechanisms of Pseudalteromonas sp. LD-B1 ' s algicidal effects on the harmful alga Heterosigma akashiwo.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,282,10.
MLA	Xu, Mingyang,et al."Investigating the molecular mechanisms of Pseudalteromonas sp. LD-B1 ' s algicidal effects on the harmful alga Heterosigma akashiwo".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 282(2024):10.