Genome-wide identification of the PP1 and PPP1R3 gene family in oyster<i> Crassostrea</i><i> gigas:</i> : Unraveling their roles in glycogen metabolism

doi:10.1016/j.aqrep.2024.102258

IOCAS-IR > 实验海洋生物学重点实验室

	Genome-wide identification of the PP1 and PPP1R3 gene family in oyster Crassostrea gigas: : Unraveling their roles in glycogen metabolism
	Zhang, Yinchuan 1,2,3,4,5; Meng, Jie2,6 ; Xu, Fei 2,3,4,5
	2024-08-01
发表期刊	AQUACULTURE REPORTS
ISSN	2352-5134
卷号	37 页码:11
通讯作者	Meng, Jie([email protected]) ; Xu, Fei([email protected])
摘要	High glycogen in oysters contributes to their flavor, quality, and hardiness, making it a crucial target trait for breeding purposes. Until now, the upstream regulatory mechanism for glycogen synthesis has remained unclear. Protein phosphatase-1 regulatory subunit 3 (PPP1R3) is a member of a family that directs the protein phosphatase-1 (PP1) to glycogen, resulting in the activation of glycogen synthase (GS) and the inactivation of glycogen phosphorylase (GP). However, their functions in molluscan species have remained unclear until now. In our study, we verified the function of the PP1 alpha/PPP1R3D pathways in the glycogen metabolism process in Crassostrea gigas. Initially, we conducted a phylogenetic analysis and identified two PP1 and PPP1R3 members in molluscan species, emphasizing copy number variations in eukaryotic animals. Additionally, we identified distinct substitutions of conserved motifs specific to bivalves, Cephalopoda, or Gastropods, revealing functional diversity in PP1 and PPP1R3. Our primary focus was on PP1 alpha/PPP1R3D in the oyster C.gigas, detected in the muscle glycogen fraction of bivalve species, with mRNA expression showing a positive correlation with glycogen content. By manipulating CgPPP1R3D expression in oysters through adenovirus-mediated overexpression or siRNA-mediated downregulation, we established a direct positive correlation between CgPPP1R3D expression levels and glycogen content. Colocation analysis confirmed protein interactions between CgPP1 alpha and CgPPP1R3D. Furthermore, to elucidate the correlation between gene expression and glycogen accumulation, we conducted an association analysis and identified a cluster of significant SNP loci upstream of CgPPP1R3D significantly related to glycogen content. The high polymorphism observed may be linked to differential expressions of CgPPP1R3D in oyster individuals with high and low glycogen levels. These findings serve as a crucial reference for enhancing glycogen traits in molluscan species.
关键词	Molluscan Glycogen Overexpression Polymorphism
DOI	10.1016/j.aqrep.2024.102258
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[32102815]; National Natural Science Foundation of China[32373157]; Key Research and Development Program of Shandong[2022LZGC015]; Taishan Scholars Program[tsqn202211250]; Youth Innovation Promotion Association of the Chinese Academy of Science[2019210]
WOS研究方向	Fisheries
WOS类目	Fisheries
WOS记录号	WOS:001276418000001
出版者	ELSEVIER
WOS关键词	TYPE-1 PROTEIN PHOSPHATASE ; REGULATORY SUBUNIT ; TARGETING SUBUNIT ; EXPRESSION ; INSULIN ; POLYMORPHISM ; VALIDATION ; DOMAIN
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186154
专题	实验海洋生物学重点实验室
通讯作者	Meng, Jie; Xu, Fei
作者单位	1.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao 266109, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 5.Shandong Technol Innovat Ctr Oyster Seed Ind, Qingdao, Peoples R China 6.Ocean Univ China, Key Lab Marine Genet & Breeding, Minist Educ, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Yinchuan,Meng, Jie,Xu, Fei. Genome-wide identification of the PP1 and PPP1R3 gene family in oyster Crassostrea gigas: : Unraveling their roles in glycogen metabolism[J]. AQUACULTURE REPORTS,2024,37:11.
APA	Zhang, Yinchuan,Meng, Jie,&Xu, Fei.(2024).Genome-wide identification of the PP1 and PPP1R3 gene family in oyster Crassostrea gigas: : Unraveling their roles in glycogen metabolism.AQUACULTURE REPORTS,37,11.
MLA	Zhang, Yinchuan,et al."Genome-wide identification of the PP1 and PPP1R3 gene family in oyster Crassostrea gigas: : Unraveling their roles in glycogen metabolism".AQUACULTURE REPORTS 37(2024):11.