Single-cell RNA-seq reveals distinct metabolic "microniches" and close host-symbiont interactions in deep-sea chemosynthetic tubeworm

doi:10.1126/sciadv.adn3053

IOCAS-IR

	Single-cell RNA-seq reveals distinct metabolic "microniches" and close host-symbiont interactions in deep-sea chemosynthetic tubeworm
	Wang, Hao 1,2; Xiao, Hongxiu 3; Feng, Buhan 3; Lan, Yi 4,5; Fung, Cheuk Wang 3; Zhang, Huan 1; Yan, Guoyong 4,5; Lian, Chao 1; Zhong, Zhaoshan1 ; Li, Jing 1; Wang, Minxiao 1; Wu, Angela Ruohao 3,6; Li, Chaolun1,7,8 ; Qian, Pei-Yuan 3,4,5
	2024-07-24
发表期刊	SCIENCE ADVANCES
ISSN	2375-2548
卷号	10 期号:30 页码:16
通讯作者	Wu, Angela Ruohao([email protected]) ; Li, Chaolun([email protected]) ; Qian, Pei-Yuan([email protected])
摘要	Vestimentiferan tubeworms that thrive in deep-sea chemosynthetic ecosystems rely on a single species of sulfide-oxidizing gammaproteobacterial endosymbionts housed in a specialized symbiotic organ called trophosome as their primary carbon source. While this simple symbiosis is remarkably productive, the host-symbiont molecular interactions remain unelucidated. Here, we applied an approach for deep-sea in situ single-cell fixation in a cold-seep tubeworm, Paraescarpia echinospica. Single-cell RNA sequencing analysis and further molecular characterizations of both the trophosome and endosymbiont indicate that the tubeworm maintains two distinct metabolic "microniches" in the trophosome by controlling the availability of chemosynthetic gases and metabolites, resulting in oxygenated and hypoxic conditions. The endosymbionts in the oxygenated niche actively conduct autotrophic carbon fixation and are digested for nutrients, while those in the hypoxic niche conduct anaerobic denitrification, which helps the host remove ammonia waste. Our study provides insights into the molecular interactions between animals and their symbiotic microbes.
DOI	10.1126/sciadv.adn3053
收录类别	SCI
语种	英语
资助项目	Science and technology innovation Project of laoshan laboratory[lSKJ202203104]; National natural Science Foundation of china[42030407]; Guangdong Government[2019B030302004]; Southern Marine Science and engineering Guangdong laboratory (Guangzhou)[2021hJ01]; Southern Marine Science and engineering Guangdong laboratory (Guangzhou)[SMSeGl20Sc01]; HKSAR government[16101822]; HKSAR government[c2013- 22G]; Chau Hoi Shuen Foundation
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:001275948300024
出版者	AMER ASSOC ADVANCEMENT SCIENCE
WOS关键词	WORM RIFTIA-PACHYPTILA ; SULFIDE BINDING ; PHYSIOLOGICAL PROTEOMICS ; CARBONIC-ANHYDRASE ; FORM-II ; INVERTEBRATE ; BLOOD ; LOCALIZATION ; SIBOGLINIDAE ; METHIONINE
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186118
专题	中国科学院海洋研究所
通讯作者	Wu, Angela Ruohao; Li, Chaolun; Qian, Pei-Yuan
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Laoshan Lab, Qingdao, Peoples R China 3.Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Peoples R China 4.Southern Marine Sci & Engn Guangdong Lab Guangzhou, Guangzhou, Peoples R China 5.Hong Kong Univ Sci & Technol, Dept Ocean Sci, Hong Kong, Peoples R China 6.Hong Kong Univ Sci & Technol, Dept Chem & Biol Engn, Hong Kong, Peoples R China 7.Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou, Peoples R China 8.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Hao,Xiao, Hongxiu,Feng, Buhan,et al. Single-cell RNA-seq reveals distinct metabolic "microniches" and close host-symbiont interactions in deep-sea chemosynthetic tubeworm[J]. SCIENCE ADVANCES,2024,10(30):16.
APA	Wang, Hao.,Xiao, Hongxiu.,Feng, Buhan.,Lan, Yi.,Fung, Cheuk Wang.,...&Qian, Pei-Yuan.(2024).Single-cell RNA-seq reveals distinct metabolic "microniches" and close host-symbiont interactions in deep-sea chemosynthetic tubeworm.SCIENCE ADVANCES,10(30),16.
MLA	Wang, Hao,et al."Single-cell RNA-seq reveals distinct metabolic "microniches" and close host-symbiont interactions in deep-sea chemosynthetic tubeworm".SCIENCE ADVANCES 10.30(2024):16.