Structural and functional characteristics of pectins from three cultivars of apple (<i> Malus</i> pumila Mill.) pomaces

doi:10.1016/j.ijbiomac.2024.132002

IOCAS-IR > 实验海洋生物学重点实验室

	*Structural and functional characteristics of pectins from three cultivars of apple ( Malus* pumila Mill.) pomaces**
	Guo, Huan 1,2; Li, Hang 3,4; Ran, Wenyi 1,2; Yu, Wenyue 1,2; Xiao, Yue 5,6; Gan, Renyou 7,8; Gao, Hong 1,2
	2024-06-01
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
卷号	269 页码:14
通讯作者	Xiao, Yue([email protected]) ; Gao, Hong([email protected])
摘要	This study aimed to investigate the chemical composition, structural properties, and biological properties of pectin polysaccharides (AP -FS, AP-QG, and AP -HG) isolated from different varieties of apple pomace. Based on the methylation and nuclear magnetic resonance analyses, the structure of AP -FS was determined to be composed of an alpha-1,4-linked homogalacturonan backbone that exhibited high levels of O-6 methylation. All pectins exhibit potent inhibitory activity against human colon cancer and human liver cancer cells, along with immunostimulatory effects. Among them, AP -FS exhibited the highest activity level. Finally, we further investigated the underlying mechanism behind the effect of AP -FS on RAW 264.7 cells using proteomics analysis. Our findings revealed that AP -FS triggers RAW 264.7 macrophage activation via NOD -like receptor (NLR), NF- kappa B, and mitogen-activated protein kinase (MAPK) signaling pathways. Therefore, our research contributes to a better understanding of the structure -function relationship among apple pectins, and AP -FS has the potential to be applied to dietary supplements targeting immunomodulation.
关键词	Apple pectin Immunostimulatory activity Structure -function relationship
DOI	10.1016/j.ijbiomac.2024.132002
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[32072321]; Sichuan University and Luzhou City Strategic Cooperation Project[2019CDLZ-20]; Liangshan Prefecture Technology Plan[21ZDYF0052]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号	WOS:001239780800001
出版者	ELSEVIER
WOS关键词	MACROPHAGE IMMUNOMODULATORY ACTIVITY ; POLYSACCHARIDE ; ESTERIFICATION ; DEGRADATION ; ANTIOXIDANT ; ACTIVATION ; FEATURES ; L.
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186024
专题	实验海洋生物学重点实验室
通讯作者	Xiao, Yue; Gao, Hong
作者单位	1.Sichuan Univ, Coll Biomass Sci & Engn, Chengdu 610065, Peoples R China 2.Sichuan Univ, Hlth Food Evaluat Res Ctr, Chengdu 610065, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 5.Sichuan Univ, West China Sch Publ Hlth, Chengdu 610041, Peoples R China 6.Sichuan Univ, West China Hosp 4, Chengdu 610041, Peoples R China 7.ASTAR, Singapore Inst Food & Biotechnol Innovat SIFBI, 31 Biopolis Way, Singapore 138669, Singapore 8.Natl Univ Singapore, Dept Food Sci & Technol, 2 Sci Dr 2, Singapore 117542, Singapore
推荐引用方式 GB/T 7714	Guo, Huan,Li, Hang,Ran, Wenyi,et al. Structural and functional characteristics of pectins from three cultivars of apple ( Malus pumila Mill.) pomaces[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,269:14.
APA	Guo, Huan.,Li, Hang.,Ran, Wenyi.,Yu, Wenyue.,Xiao, Yue.,...&Gao, Hong.(2024).Structural and functional characteristics of pectins from three cultivars of apple ( Malus pumila Mill.) pomaces.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,269,14.
MLA	Guo, Huan,et al."Structural and functional characteristics of pectins from three cultivars of apple ( Malus pumila Mill.) pomaces".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 269(2024):14.