Gene structure, expression and function analysis of the MyoD gene in the Pacific white shrimp Litopenaeus vannamei

doi:10.1016/j.gene.2024.148523

IOCAS-IR > 实验海洋生物学重点实验室

	Gene structure, expression and function analysis of the MyoD gene in the Pacific white shrimp Litopenaeus vannamei
	Xia, Yanting 1,2,3; Zhang, Xiaojun 1,2,3,4,5; Zhang, Xiaoxi1,2 ; Zhu, Haochen 1,2,3; Zhong, Xiaoyun 1,2,4; Song, Weixiao 6; Yuan, Jianbo 1,2,4,5; Sha, Zhenxia 3; Li, Fuhua 1,2,4,5
	2024-08-30
发表期刊	GENE
ISSN	0378-1119
卷号	921 页码:14
通讯作者	Zhang, Xiaojun([email protected])
摘要	The Pacific white shrimp Litopenaeus vannamei is a representative species of decapod crustacean and an economically important marine aquaculture species worldwide. However, research on the genes involved in muscle growth and development in shrimp is still lacking. MyoD is recognized as a crucial regulator of myogenesis and plays an essential role in muscle growth and differentiation in various animals. Nonetheless, little information is available concerning the function of this gene among crustaceans. In this study, we identified a sequence of the MyoD gene (LvMyoD) with a conserved bHLH domain in the L. vannamei genome. Phylogenetic analysis revealed that both the overall protein sequence and specific functional sites of LvMyoD are highly conserved with those of other crustacean species and that they are evolutionarily closely related to vertebrate MyoD and Myf5. LvMyoD expression is initially high during early muscle development in shrimp and gradually decreases after 40 days post-larval development. In adults, the muscle-specific expression of LvMyoD was confirmed through RT-qPCR analysis. Knockdown of LvMyoD inhibited the growth of the shrimp in body length and weight. Histological observation and transcriptome sequencing of muscle samples after RNA interference (RNAi) revealed nuclear agglutination and looseness in muscle fibers. Additionally, we observed significant effects on the expression of genes involved in heat shock proteins, myosins, actins, protein synthesis, and glucose metabolism. These findings suggest that LvMyoD plays a critical role in regulating muscle protein synthesis and muscle cell differentiation. Overall, this study highlights the involvement of LvMyoD in myogenesis and muscle growth, suggesting that it is a potentially important regulatory target for shrimp breeding efforts.
关键词	MyoD gene Litopenaeus vannamei RNA interference Expression Growth
DOI	10.1016/j.gene.2024.148523
收录类别	SCI
语种	英语
资助项目	National Natural Sciences Foundation of China[32273102]; National Key R &D Program of China[2022YFF1000304]; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030105]; Taishan Scholars Program; Oceanographic Data Center, IOCAS
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:001240765500001
出版者	ELSEVIER
WOS关键词	HEAT-SHOCK PROTEINS ; MUSCLE DIFFERENTIATION ; RYANODINE RECEPTORS ; MYOGENIC FACTOR ; TRANSCRIPTION ; DROSOPHILA ; FAMILY ; FIBROBLASTS ; NAUTILUS ; CELLS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186012
专题	实验海洋生物学重点实验室
通讯作者	Zhang, Xiaojun
作者单位	1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Inst Oceanol, Chinese Acad Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Univ, Inst Aquat Biotechnol, Collage Life Sci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan 430072, Peoples R China 6.Univ British Columbia, Vancouver, BC, Canada
推荐引用方式 GB/T 7714	Xia, Yanting,Zhang, Xiaojun,Zhang, Xiaoxi,et al. Gene structure, expression and function analysis of the MyoD gene in the Pacific white shrimp Litopenaeus vannamei[J]. GENE,2024,921:14.
APA	Xia, Yanting.,Zhang, Xiaojun.,Zhang, Xiaoxi.,Zhu, Haochen.,Zhong, Xiaoyun.,...&Li, Fuhua.(2024).Gene structure, expression and function analysis of the MyoD gene in the Pacific white shrimp Litopenaeus vannamei.GENE,921,14.
MLA	Xia, Yanting,et al."Gene structure, expression and function analysis of the MyoD gene in the Pacific white shrimp Litopenaeus vannamei".GENE 921(2024):14.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
1-s2.0-S037811192400（7340KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览