Sodium selenite enhances the production of functional proteins and biomass in Chlorella pyrenoidosa 038F by promoting acetate assimilation under heterotrophic cultivation

doi:10.1016/j.aquaculture.2024.740987

IOCAS-IR > 实验海洋生物学重点实验室

	Sodium selenite enhances the production of functional proteins and biomass in Chlorella pyrenoidosa 038F by promoting acetate assimilation under heterotrophic cultivation
	Shangguan, Jingjing 1,2,3; Yang, Na 1,2; Zhang, Litao 1,2; Liu, Jianguo 1,2,5; Li, Yueming 4; Xu, Jianchun 4; Xia, Xiuluan 4; Xu, Bingzheng 4
	2024-08-30
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	589 页码:10
通讯作者	Liu, Jianguo([email protected])
摘要	Unicellular microalga Chlorella pyrenoidosa is an excellent source of protein, but its ability to produce functional proteins has received less attention. In this study, a strategy to enhance the levels of functional proteins and biomass, by adding sodium selenite under heterotrophic cultivation, is reported for C. pyrenoidosa 038F. Activities of catalase, superoxide dismutase, and glutathione peroxidase were significantly enhanced by 1.94-, 1.90and 32.73-fold, respectively, in C. pyrenoidosa 038F exposed to 20 mg/L of sodium selenite. Further, western blot analysis confirmed that sodium selenite promoted the synthesis of glutathione peroxidase, which might be due to the up-regulation of peroxisome and selenocysteine-synthesis-related genes. Sodium selenite also increased biomass, the content of protein and soluble sugar by 1.32-, 1.29-, 1.46-fold, respectively. Subsequent transcriptomic analysis confirmed that cellular respiration and the gluconeogenesis pathway were enhanced. This study highlights the potential use of heterotrophic C. pyrenoidosa 038F as a novel selenoprotein-rich carrier while simultaneously producing soluble sugar and biomass. These results might be helpful for increasing economic production of C. pyrenoidosa under heterotrophic cultivation.
关键词	Glutathione peroxidase Chlorella pyrenoidosa Sodium selenite Carbon metabolism Transcriptome Gluconeogenesis
DOI	10.1016/j.aquaculture.2024.740987
收录类别	SCI
语种	英语
资助项目	TS Industrial Experts Programme[LJNY202102]; Strategic Priority Research Program of the Chinese Academy of Sciences, China[XDB42000000]; Science & Technology Specific Project in Agricultural High-tech Industrial Demonstration Area of the Yellow River Delta[2022SZX13]
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:001235420600001
出版者	ELSEVIER
WOS关键词	GROWTH ; RESPIRATION ; STRESS ; PLANTS
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/185988
专题	实验海洋生物学重点实验室
通讯作者	Liu, Jianguo
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, 168 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Qingdao Langyatai Grp Co Ltd, Qingdao 266500, Peoples R China 5.Natl Ctr Technol Innovat Comprehens Utilizat Salin, Acad Workstat Agr High Tech Ind Area Yellow River, Dongying 257300, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Shangguan, Jingjing,Yang, Na,Zhang, Litao,et al. Sodium selenite enhances the production of functional proteins and biomass in Chlorella pyrenoidosa 038F by promoting acetate assimilation under heterotrophic cultivation[J]. AQUACULTURE,2024,589:10.
APA	Shangguan, Jingjing.,Yang, Na.,Zhang, Litao.,Liu, Jianguo.,Li, Yueming.,...&Xu, Bingzheng.(2024).Sodium selenite enhances the production of functional proteins and biomass in Chlorella pyrenoidosa 038F by promoting acetate assimilation under heterotrophic cultivation.AQUACULTURE,589,10.
MLA	Shangguan, Jingjing,et al."Sodium selenite enhances the production of functional proteins and biomass in Chlorella pyrenoidosa 038F by promoting acetate assimilation under heterotrophic cultivation".AQUACULTURE 589(2024):10.