Different Immune Responses of Hemocytes from <i>V. parahaemolyticus</i>-Resistant and -Susceptible Shrimp at Early Infection Stage

doi:10.3390/biology13050300

IOCAS-IR > 实验海洋生物学重点实验室

	*Different Immune Responses of Hemocytes from V. parahaemolyticus-Resistant and -Susceptible Shrimp at Early Infection Stage*
	Du, Wenran 1,2,3,4; Li, Shihao 2,3,4,5,6; Li, Fuhua 2,3,4,5,6
	2024-05-01
发表期刊	BIOLOGY-BASEL
卷号	13 期号:5 页码:12
通讯作者	Li, Shihao([email protected])
摘要	Vibrio parahaemolyticus is one of the main causative agents leading to acute hepatopancreatic necrosis disease, the severe bacterial disease that occurs during shrimp aquaculture. Hemocytes play important roles during Vibrio infection. Previously, we found that there were few differentially expressed genes (DEGs) between hemocytes from V. parahaemolyticus-resistant and -susceptible shrimp before infection. We considered that there should be different immune responses between them after a pathogen infection. Here, the transcriptome data of hemocytes from V. parahaemolyticus-resistant and -susceptible shrimp before and after a pathogen infection were compared. The results showed that there were 157 DEGs responsive to infection in V. parahaemolyticus-resistant shrimp, while 33 DEGs in V. parahaemolyticus-susceptible shrimp. DEGs in V. parahaemolyticus-resistant shrimp were mainly related to immune and glycolytic processes, while those in V. parahaemolyticus-susceptible shrimp were mainly related to metabolism, with only two DEGs in common. A further analysis of genes involved in glucose metabolism revealed that GLUT2, HK, FBP, and PCK1 were lowly expressed while PC were highly expressed in hemocytes of the V. parahaemolyticus-resistant shrimp, indicating that glucose metabolism in shrimp hemocytes was related to a V. parahaemolyticus infection. After the knockdown of PC, the expression of genes in Toll and IMD signaling pathways were down-regulated, indicating that glucose metabolism might function through regulating host immunity during V. parahaemolyticus infection. The results suggest that the immune responses between V. parahaemolyticus-resistant and -susceptible shrimp were apparently different, which probably contribute to their different V. parahaemolyticus resistance abilities.
关键词	penaeid shrimp hemocytes Vibrio parahaemolyticus glycolysis immunity
DOI	10.3390/biology13050300
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS类目	Biology
WOS记录号	WOS:001233527400001
出版者	MDPI
WOS关键词	EXPRESSION ; TREHALOSE ; STRINGTIE ; HISAT
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/185973
专题	实验海洋生物学重点实验室
通讯作者	Li, Shihao
作者单位	1.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China 2.Chinese Acad Sci, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 5.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan 430072, Peoples R China 6.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Du, Wenran,Li, Shihao,Li, Fuhua. Different Immune Responses of Hemocytes from V. parahaemolyticus-Resistant and -Susceptible Shrimp at Early Infection Stage[J]. BIOLOGY-BASEL,2024,13(5):12.
APA	Du, Wenran,Li, Shihao,&Li, Fuhua.(2024).Different Immune Responses of Hemocytes from V. parahaemolyticus-Resistant and -Susceptible Shrimp at Early Infection Stage.BIOLOGY-BASEL,13(5),12.
MLA	Du, Wenran,et al."Different Immune Responses of Hemocytes from V. parahaemolyticus-Resistant and -Susceptible Shrimp at Early Infection Stage".BIOLOGY-BASEL 13.5(2024):12.