Characterization of the Apoptotic and Antimicrobial Activities of Two Initiator Caspases of Sea Cucumber <i>Apostichopus japonicus</i>

doi:10.3390/genes15050540

IOCAS-IR > 实验海洋生物学重点实验室

	Characterization of the Apoptotic and Antimicrobial Activities of Two Initiator Caspases of Sea Cucumber Apostichopus japonicus
	Zhu, Hanshuo1,2,3 ; Yuan, Zihao1,2,3 ; Xu, Hang1 ; Sun, Li 1,2,3
	2024-05-01
发表期刊	GENES
卷号	15 期号:5 页码:13
通讯作者	Xu, Hang([email protected]) ; Sun, Li([email protected])
摘要	Caspase (CASP) is a protease family that plays a vital role in apoptosis, development, and immune response. Herein, we reported the identification and characterization of two CASPs, AjCASPX1 and AjCASPX2, from the sea cucumber Apostichopus japonicus, an important aquaculture species. AjCASPX1/2 share similar domain organizations with the vertebrate initiator caspases CASP2/9, including the CARD domain and the p20/p10 subunits with conserved functional motifs. However, compared with human CASP2/9, AjCASPX1/2 possess unique structural features in the linker region between p20 and p10. AjCASPX1, but not AjCASPX2, induced marked apoptosis of human cells by activating CASP3/7. The recombinant proteins of AjCASPX2 and the CARD domain of AjCASPX2 were able to bind to a wide range of bacteria, as well as bacterial cell wall components, and inhibit bacterial growth. AjCASPX1, when expressed in Escherichia coli, was able to kill the host bacteria. Under normal conditions, AjCASPX1 and AjCASPX2 expressions were most abundant in sea cucumber muscle and coelomocytes, respectively. After bacterial infection, both AjCASPX1 and AjCASPX2 expressions were significantly upregulated in sea cucumber tissues and cells. Together, these results indicated that AjCASPX1 and AjCASPX2 were initiator caspases with antimicrobial activity and likely functioned in apoptosis and immune defense against pathogen infection.
关键词	Apostichopus japonicus caspase apoptosis antimicrobial
DOI	10.3390/genes15050540
收录类别	SCI
语种	英语
资助项目	Science & Technology Innovation Project of Laoshan Laboratory
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:001232384200001
出版者	MDPI
WOS关键词	ACTIVATION ; CELOMOCYTES ; MECHANISMS ; AUTOLYSIS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/185937
专题	实验海洋生物学重点实验室
通讯作者	Xu, Hang; Sun, Li
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266404, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Coll Marine Sci, Qingdao 266404, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhu, Hanshuo,Yuan, Zihao,Xu, Hang,et al. Characterization of the Apoptotic and Antimicrobial Activities of Two Initiator Caspases of Sea Cucumber Apostichopus japonicus[J]. GENES,2024,15(5):13.
APA	Zhu, Hanshuo,Yuan, Zihao,Xu, Hang,&Sun, Li.(2024).Characterization of the Apoptotic and Antimicrobial Activities of Two Initiator Caspases of Sea Cucumber Apostichopus japonicus.GENES,15(5),13.
MLA	Zhu, Hanshuo,et al."Characterization of the Apoptotic and Antimicrobial Activities of Two Initiator Caspases of Sea Cucumber Apostichopus japonicus".GENES 15.5(2024):13.