Extensive intragenomic variations of the 18S rDNA V4 region in the toxigenic diatom species <i>Pseudo-nitzschia</i> multistriata revealed through high-throughput sequencing

doi:10.1016/j.marpolbul.2024.116198

	*Extensive intragenomic variations of the 18S rDNA V4 region in the toxigenic diatom species Pseudo-nitzschia* multistriata revealed through high-throughput sequencing**
	Wang, Hui1,2,3,4 ; Liu, Kuiyan 1,2,3,4; Chen, Ziyan 1,2,3,4; Chen, Yang1,2,3,4 ; Hu, Zhangxi5 ; Chen, Weizhou 6; Leaw, Chui Pin 7; Chen, Nansheng 1,2,4,8
	2024-04-01
发表期刊	MARINE POLLUTION BULLETIN
ISSN	0025-326X
卷号	201 页码:11
通讯作者	Chen, Nansheng([email protected])
摘要	Metabarcoding analysis is an effective technique for monitoring the domoic acid-producing Pseudo-nitzschia species in marine environments, uncovering high-levels of molecular diversity. However, such efforts may result in the overinterpretation of Pseudo-nitzschia species diversity, as molecular diversity not only encompasses interspecies and intraspecies diversities but also exhibits extensive intragenomic variations (IGVs). In this study, we analyzed the V4 region of the 18S rDNA of 30 strains of Pseudo-nitzschia multistriata collected from the coasts of China. The results showed that each P. multistriata strain harbored about a hundred of unique 18S rDNA V4 sequence varieties, of which each represented by a unique amplicon sequence variant (ASV). This study demonstrated the extensive degree of IGVs in P. multistriata strains, suggesting that IGVs may also present in other Pseudo-nitzschia species and other phytoplankton species. Understanding the scope and levels of IGVs is crucial for accurately interpreting the results of metabarcoding analysis.
关键词	Metabarcoding analysis Pseudo-nitzschia multistriata Molecular markers polymorphism SSU rDNA Intragenomic variations (IGVs)
DOI	10.1016/j.marpolbul.2024.116198
收录类别	SCI
语种	英语
资助项目	National Key Research and Development (R&D) Program of China[2022YFC3105200]; Taishan Scholar Project Special Fund; Natural Science Foundation of China[42176162]; Strategic Priority Research Program of Chinese Academy of Sciences[XDB42000000]; Chinese Academy of Sciences Pioneer Hundred Talents Program
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS类目	Environmental Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:001220642900001
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS关键词	RIBOSOMAL-RNA GENES ; DOMOIC ACID ; PHYLOGENETIC-RELATIONSHIPS ; MARINE PROTISTS ; SP-NOV ; BACILLARIOPHYCEAE ; DIVERSITY ; DNA ; MORPHOLOGY ; TAXONOMY
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/185690
专题	海洋生态与环境科学重点实验室
通讯作者	Chen, Nansheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China 3.Univ Chinese Acad Sci, Coll Marine Sci, Beijing 100039, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Guangdong Ocean Univ, Coll Fisheries, Dept Aquaculture, Zhanjiang 524088, Guangdong, Peoples R China 6.Shantou Univ, Inst Marine Biol, Shantou 515063, Guangdong, Peoples R China 7.Univ Malaya, Inst Ocean & Earth Sci, Bachok Marine Res Stn, Bachok 16310, Kelantan, Malaysia 8.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Wang, Hui,Liu, Kuiyan,Chen, Ziyan,et al. Extensive intragenomic variations of the 18S rDNA V4 region in the toxigenic diatom species Pseudo-nitzschia multistriata revealed through high-throughput sequencing[J]. MARINE POLLUTION BULLETIN,2024,201:11.
APA	Wang, Hui.,Liu, Kuiyan.,Chen, Ziyan.,Chen, Yang.,Hu, Zhangxi.,...&Chen, Nansheng.(2024).Extensive intragenomic variations of the 18S rDNA V4 region in the toxigenic diatom species Pseudo-nitzschia multistriata revealed through high-throughput sequencing.MARINE POLLUTION BULLETIN,201,11.
MLA	Wang, Hui,et al."Extensive intragenomic variations of the 18S rDNA V4 region in the toxigenic diatom species Pseudo-nitzschia multistriata revealed through high-throughput sequencing".MARINE POLLUTION BULLETIN 201(2024):11.