Transient receptor potential (TRP) channels in Sebastes schlegelii: Genome-wide identification and ThermoTRP expression analysis under high-temperature

doi:10.1016/j.gene.2024.148317

IOCAS-IR > 实验海洋生物学重点实验室

	Transient receptor potential (TRP) channels in Sebastes schlegelii: Genome-wide identification and ThermoTRP expression analysis under high-temperature
	Huang, Jinwei 1,2,3,4; Wang, Xueying1,2,3 ; Guo, Xiaoyang 1,2,3,5; Liu, Qinghua 1,2,3; Li, Jun 1,2,3
	2024-06-05
发表期刊	GENE
ISSN	0378-1119
卷号	910 页码:12
通讯作者	Wang, Xueying([email protected]) ; Li, Jun([email protected])
摘要	Transient Receptor Potential (TRP) channels, essential for sensing environmental stimuli, are widely distributed. Among them, thermosensory TRP channels play a crucial role in temperature sensing and regulation. Sebastes schlegelii, a significant aquatic economic species, exhibits sensitivity to temperature across multiple aspects. In this study, we identified 18 SsTRP proteins using whole-genome scanning. Motif analysis revealed motif 2 in all TRP proteins, with conserved motifs in subfamilies. TRP-related domains, anchored repeats, and ion-transmembrane domains were found. Chromosome analysis showed 18 TRP genes on 11 chromosomes and a scaffold. Phylogenetics classified SsTRPs into four subfamilies: TRPM, TRPA, TRPV, and TRPC. In diverse organisms, four monophyletic subfamilies were identified. Additionally, we identified key TRP genes with significantly upregulated transcription levels under short-term (30 min) and long-term (3 days) exposure at 24 degrees C (optimal elevated temperature) and 27 degrees C (critical high temperature). We propose that genes upregulated at 30 min may be involved in the primary response process of temperature sensing, while genes upregulated at 3 days may participate in the secondary response process of temperature perception. This study lays the foundation for understanding the regulatory mechanisms of TRPs responses to environmental stimuli in S. schlegelii and other fishes.
关键词	Sebastes schlegelii Transient receptor potential (TRP) channels Temperature
DOI	10.1016/j.gene.2024.148317
收录类别	SCI
语种	英语
资助项目	China Agriculture Research System[CARS -47]; National Marine Genetic Resource Center
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:001203720500001
出版者	ELSEVIER
WOS关键词	ION-CHANNEL ; THERMAL SENSITIVITY ; CAPSAICIN-RECEPTOR ; CATION CHANNEL ; REEF FISHES ; HEAT ; REPRODUCTION ; PERFORMANCE ; ACTIVATION ; MORTALITY
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/185550
专题	实验海洋生物学重点实验室
通讯作者	Wang, Xueying; Li, Jun
作者单位	1.Chinese Acad Sci, CAS, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Huang, Jinwei,Wang, Xueying,Guo, Xiaoyang,et al. Transient receptor potential (TRP) channels in Sebastes schlegelii: Genome-wide identification and ThermoTRP expression analysis under high-temperature[J]. GENE,2024,910:12.
APA	Huang, Jinwei,Wang, Xueying,Guo, Xiaoyang,Liu, Qinghua,&Li, Jun.(2024).Transient receptor potential (TRP) channels in Sebastes schlegelii: Genome-wide identification and ThermoTRP expression analysis under high-temperature.GENE,910,12.
MLA	Huang, Jinwei,et al."Transient receptor potential (TRP) channels in Sebastes schlegelii: Genome-wide identification and ThermoTRP expression analysis under high-temperature".GENE 910(2024):12.