| Sensitive and Rapid Detection of Genetic Modified Soybean (Roundup Ready) by Loop-Mediated Isothermal Amplification | |
| Liu, Mei1; Luo, Yan2; Tao, Ran1,3; He, Ru4; Jiang, Keyong1; Wang, Baojie1; Wang, Lei1; Wang, L, Chinese Acad Sci, R&D Ctr Marine Biotechnol, Inst Oceanol, Qingdao 266071, Peoples R China | |
| 2009-11-01 | |
| 发表期刊 | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
 |
| ISSN | 0916-8451 |
| 卷号 | 73期号:11页码:2365-2369 |
| 文章类型 | Article |
| 摘要 | Using the LAMP method, a highly specific and sensitive detection system for genetically modified soybean (Roundup Ready) was designed. In this detection system, a set of four primers was designed by targeting the exogenous 35S epsps gene. Target DNA was amplified and visualized on agarose gel within 45 min under isothermal conditions at 65 degrees C. Without gel electrophoresis, the LAMP amplicon was visualized directly in the reaction tube by the addition of SYBR Green I for naked-eye inspection. The detection sensitivity of LAMP was 10-fold higher than the nested PCR established in our laboratory. Moreover, the LAMP method was much quicker, taking only 70 min, as compared with 300 min for nested PCR to complete the analysis of the GM soybean. Compared with traditional PCR approaches, the LAMP procedure is faster and more sensitive, and there is no need for a special PCR machine or electrophoresis equipment. Hence, this method can be a very useful tool for GMO detection and is particularly convenient for fast screening.; Using the LAMP method, a highly specific and sensitive detection system for genetically modified soybean (Roundup Ready) was designed. In this detection system, a set of four primers was designed by targeting the exogenous 35S epsps gene. Target DNA was amplified and visualized on agarose gel within 45 min under isothermal conditions at 65 degrees C. Without gel electrophoresis, the LAMP amplicon was visualized directly in the reaction tube by the addition of SYBR Green I for naked-eye inspection. The detection sensitivity of LAMP was 10-fold higher than the nested PCR established in our laboratory. Moreover, the LAMP method was much quicker, taking only 70 min, as compared with 300 min for nested PCR to complete the analysis of the GM soybean. Compared with traditional PCR approaches, the LAMP procedure is faster and more sensitive, and there is no need for a special PCR machine or electrophoresis equipment. Hence, this method can be a very useful tool for GMO detection and is particularly convenient for fast screening. |
| 关键词 | Loop-mediated Isothermal Amplification Roundup Ready (Rr) Soybean Genetically Modified Organism (Gmo) Detection |
| 学科领域 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry, Applied ; Food Science & Technology |
| DOI | 10.1271/bbb.80723 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000272319900001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/1854 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Wang, L, Chinese Acad Sci, R&D Ctr Marine Biotechnol, Inst Oceanol, Qingdao 266071, Peoples R China |
| 作者单位 | 1.Chinese Acad Sci, R&D Ctr Marine Biotechnol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Ocean Univ China, Sch Life Sci, Qingdao 266003, Peoples R China 3.Rongcheng Entry Exit Inspect & Quarantine Bur, Rongcheng 264300, Peoples R China 4.Haerbin Univ Ind, Weihai Branch, Weihai 264209, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Liu, Mei,Luo, Yan,Tao, Ran,et al. Sensitive and Rapid Detection of Genetic Modified Soybean (Roundup Ready) by Loop-Mediated Isothermal Amplification[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2009,73(11):2365-2369. |
| APA | Liu, Mei.,Luo, Yan.,Tao, Ran.,He, Ru.,Jiang, Keyong.,...&Wang, L, Chinese Acad Sci, R&D Ctr Marine Biotechnol, Inst Oceanol, Qingdao 266071, Peoples R China.(2009).Sensitive and Rapid Detection of Genetic Modified Soybean (Roundup Ready) by Loop-Mediated Isothermal Amplification.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,73(11),2365-2369. |
| MLA | Liu, Mei,et al."Sensitive and Rapid Detection of Genetic Modified Soybean (Roundup Ready) by Loop-Mediated Isothermal Amplification".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 73.11(2009):2365-2369. |
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