Antibacterial insights into alternariol and its derivative alternariol monomethyl ether produced by a marine fungus

doi:10.1128/aem.00058-24

IOCAS-IR > 实验海洋生物学重点实验室

	Antibacterial insights into alternariol and its derivative alternariol monomethyl ether produced by a marine fungus
	Li, Rongmei 1,2,3,4,5; Su, Zhenjie 2,3,4,5,6; Sun, Chaomin 2,3,4,5,6; Wu, Shimei 1
	2024-04-17
发表期刊	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN	0099-2240
卷号	90 期号:4 页码:25
通讯作者	Sun, Chaomin([email protected]) ; Wu, Shimei([email protected])
摘要	Alternaria alternata FB1 is a marine fungus identified as a candidate for plastic degradation in our previous study. This fungus has been recently shown to produce secondary metabolites with significant antimicrobial activity against various pathogens, including methicillin-resistant Staphylococcus aureus (MRSA) and the notorious aquaculture pathogen Vibrio anguillarum. The antibacterial compounds were purified and identified as alternariol (AOH) and its derivative, alternariol monomethyl ether (AME). We found that AOH and AME primarily inhibited pathogenic bacteria (MRSA or V. anguillarum) by disordering cell division and some other key physiological and biochemical processes. We further demonstrated that AOH could effectively inhibit the unwinding activity of MRSA topoisomerases, which are closely related to cell division and are the potential action target of AOH. The antibacterial activities of AOH and AME were verified by using zebrafish as the in vivo model. Notably, AOH and AME did not significantly affect the viability of normal human liver cells at concentrations that effectively inhibited MRSA or V. anguillarum. Finally, we developed the genetic operation system of A. alternata FB1 and blocked the biosynthesis of AME by knocking out omtI (encoding an O-methyl transferase), which facilitated A. alternata FB1 to only produce AOH. The development of this system in the marine fungus will accelerate the discovery of novel natural products and further bioactivity study. IMPORTANCE More and more scientific reports indicate that alternariol (AOH) and its derivative alternariol monomethyl ether (AME) exhibit antibacterial activities. However, limited exploration of their detailed antibacterial mechanisms has been performed. In the present study, the antibacterial mechanisms of AOH and AME produced by the marine fungus Alternaria alternata FB1 were disclosed in vitro and in vivo. Given their low toxicity on the normal human liver cell line under the concentrations exhibiting significant antibacterial activity against different pathogens, AOH and AME are proposed to be good candidates for developing promising antibiotics against methicillin-resistant Staphylococcus aureus and Vibrio anguillarum. We also succeeded in blocking the biosynthesis of AME, which facilitated us to easily obtain pure AOH. Moreover, based on our previous results, A. alternata FB1 was shown to enable polyethylene degradation.
关键词	antibacteria biosynthesis alternariol and derivative marine fungus multidrug-resistant bacteria
DOI	10.1128/aem.00058-24
收录类别	SCI
语种	英语
资助项目	Science and Technology Innovation Project of Laoshan Laboratory; Shandong Provincial Natural Science Foundation[ZR2021ZD28]; NSFC Innovative Group Grant[42221005]; National Natural Science Foundation of China[92351301]; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301]; Key Collaborative Research Program of the Alliance of International Science Organizations; Key deployment projects of Center of Ocean Mega-Science of the Chinese Academy of Sciences[COMS2020Q04]; Taishan Scholars Program[tstp20230637]; [2022QNLM030004-3]; [LSKJ202203103]
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:001183409600001
出版者	AMER SOC MICROBIOLOGY
WOS关键词	ENDOPHYTIC FUNGUS ; INHIBITORS ; ALKALOIDS ; STRESS
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/185068
专题	实验海洋生物学重点实验室深海极端环境与生命过程研究中心
通讯作者	Sun, Chaomin; Wu, Shimei
作者单位	1.Qingdao Univ, Coll Life Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China 4.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 6.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所; 深海极端环境与生命过程研究中心
通讯作者单位	中国科学院海洋研究所; 深海极端环境与生命过程研究中心
推荐引用方式 GB/T 7714	Li, Rongmei,Su, Zhenjie,Sun, Chaomin,et al. Antibacterial insights into alternariol and its derivative alternariol monomethyl ether produced by a marine fungus[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2024,90(4):25.
APA	Li, Rongmei,Su, Zhenjie,Sun, Chaomin,&Wu, Shimei.(2024).Antibacterial insights into alternariol and its derivative alternariol monomethyl ether produced by a marine fungus.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,90(4),25.
MLA	Li, Rongmei,et al."Antibacterial insights into alternariol and its derivative alternariol monomethyl ether produced by a marine fungus".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 90.4(2024):25.