Morphological and molecular analyses of a <i>Philine kinglipini</i> outbreak in summer of 2022 in Jiaozhou Bay, China

doi:10.1007/s00343-023-3037-1

	*Morphological and molecular analyses of a Philine kinglipini* outbreak in summer of 2022 in Jiaozhou Bay, China**
	Duan, Zelin 1,2,3; Yu, Yanan 1,3,4; Wang, Jing1,2,3 ; Zhang, Junlong1,3,4,5 ; Chen, Nansheng 1,2,3,6
	2024-02-22
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
页码	18
通讯作者	Chen, Nansheng([email protected])
摘要	A sudden and unprecedented outbreak of molluscan Philine kinglipini occurred in summer 2022 in Jiaozhou Bay, Shandong, China, causing substantial damage to local mariculture industry of the Manila clam Ruditapes philippinarum. Although P. kinglipini has been found in many coastal regions of China, the molecular research of P. kinglipini has not been extensively studied, making it difficult to accurately identify and track P. kinglipini samples in field using molecular methods. Samples were collected during the outbreak and their morphological features and molecular sequences were analyzed. Results show that the causative species of the outbreak was P. kinglipini. The mitochondrial genome (mtDNA) of P. kinglipini was constructed for the first time, based on which phylogenetic analysis of the mtDNAs of P. kinglipini and related species in the order Cephalaspidea was carried out. As revealed by metabarcoding analysis of 18S rDNA V4, the seasonal change of P. kinglipini and closely related Philine species was striking with peaks between April and August. Therefore, metabarcoding analysis is applicable tool for monitoring the bloom development of P. kinglipini and related species. This study generated for the first time essential molecular marker sequences and mtDNA of P. kinglipini, which provided a reference for future characterization and monitoring of its outbreaks and for phylogenetic analysis of Philine species.
关键词	marine species outbreaks metabarcoding analysis mitochondrial genome Philine kinglipini phylogenetics spatial-temporal dynamics
DOI	10.1007/s00343-023-3037-1
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050302]; Strategic Priority Research Program of Chinese Academy of Sciences[XDB42000000]; Taishan Scholar Project Special Fund; Postdoctoral Program of Qingdao[E2KY14101N]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:001174418800002
出版者	SCIENCE PRESS
WOS关键词	ENVIRONMENTAL DNA ; COMMUNITY STRUCTURE ; TOOL ; MACROBENTHOS ; ALIGNMENT ; WATERS ; PERFORMANCE ; EMPHASIS ; MOLLUSCA ; TREES
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/185011
专题	海洋生态与环境科学重点实验室海洋生物分类与系统演化实验室
通讯作者	Chen, Nansheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Laoshan Lab, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao Key Lab Marine Biodivers & Conservat, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Marine Biol Museum, Qingdao 266071, Peoples R China 6.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Duan, Zelin,Yu, Yanan,Wang, Jing,et al. Morphological and molecular analyses of a Philine kinglipini outbreak in summer of 2022 in Jiaozhou Bay, China[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2024:18.
APA	Duan, Zelin,Yu, Yanan,Wang, Jing,Zhang, Junlong,&Chen, Nansheng.(2024).Morphological and molecular analyses of a Philine kinglipini outbreak in summer of 2022 in Jiaozhou Bay, China.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,18.
MLA	Duan, Zelin,et al."Morphological and molecular analyses of a Philine kinglipini outbreak in summer of 2022 in Jiaozhou Bay, China".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2024):18.