Fucoidan from the cell wall of<i> Silvetia</i><i> siliquosa</i> with immunomodulatory effect on RAW 264.7 cells

doi:10.1016/j.carbpol.2024.121883

IOCAS-IR > 实验海洋生物学重点实验室

	Fucoidan from the cell wall of Silvetia siliquosa with immunomodulatory effect on RAW 264.7 cells
	Geng, Lihua 1,2,3,4; Zhang, Quanbin 1,2,3,4; Li, Qiong5 ; Zhang, Qian 4,6; Wang, Cong 7; Song, Ni 7; Xin, Wenyu 8
	2024-05-15
发表期刊	CARBOHYDRATE POLYMERS
ISSN	0144-8617
卷号	332 页码:13
通讯作者	Xin, Wenyu([email protected])
摘要	Silvetia siliquosa, the only species of the family Fucaceae in China, is used as a medicine food homology. Fucoidan from S. siliquosa was extracted by hot water twice thoroughly (13 % of total yield), and a purified fucoidan SSF with a molecular weight of 93 kD was obtained. Chemical composition analysis demonstrated that SSF was primarily composed of sulfate (21.68 wt%) and fucose (84 % of all neutral monosaccharides). IR, methylation analysis, NMR and ESI-MS results indicated SSF had the backbone of mainly (1 -* 3)-alpha-L-fucopyranose and minor (1 -* 4)-alpha-L-fucopyranose, with little 1,3 and 1,4 branched beta-D-Xylp and beta-D-Galp. The in vitro immunomodulatory test on RAW 264.7 cells showed that SSF could up-regulate the expression of immune related factors and proteins in a concentration-dependent manner, but the immunomodulatory effect disappeared from desulfated SSF. This research indicated that highly sulfated fucan possessed immunomodulatory effect and the importance of sulfate groups in the activity of SSF.
关键词	Silvetia siliquosa Fucoidan Polysaccharide Immunomodulatory activity Sulfate groups
DOI	10.1016/j.carbpol.2024.121883
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[32201049]
WOS研究方向	Chemistry ; Polymer Science
WOS类目	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS记录号	WOS:001183973800001
出版者	ELSEVIER SCI LTD
WOS关键词	SACCHARINA-JAPONICA ; BROWN SEAWEED ; STRUCTURAL CHARACTERISTICS ; SULFATED POLYSACCHARIDES ; EXTRACTION ; HETEROPOLYSACCHARIDE ; FUCOSTEROL ; ALGAE
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184990
专题	实验海洋生物学重点实验室
通讯作者	Xin, Wenyu
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Inst Oceanol, Chinese Acad Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 5.Shandong Marine Forecast & Hazard Mitigat Serv, Qingdao, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, Publ Technol Serv Ctr, Qingdao, Peoples R China 7.Ocean Univ China, Sch Med & Pharm, Qingdao, Peoples R China 8.Binzhou Med Univ, Sch Pharm, Key Lab Prescript Effect & Clin Evaluat State Adm, Yantai, Shandong, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Geng, Lihua,Zhang, Quanbin,Li, Qiong,et al. Fucoidan from the cell wall of Silvetia siliquosa with immunomodulatory effect on RAW 264.7 cells[J]. CARBOHYDRATE POLYMERS,2024,332:13.
APA	Geng, Lihua.,Zhang, Quanbin.,Li, Qiong.,Zhang, Qian.,Wang, Cong.,...&Xin, Wenyu.(2024).Fucoidan from the cell wall of Silvetia siliquosa with immunomodulatory effect on RAW 264.7 cells.CARBOHYDRATE POLYMERS,332,13.
MLA	Geng, Lihua,et al."Fucoidan from the cell wall of Silvetia siliquosa with immunomodulatory effect on RAW 264.7 cells".CARBOHYDRATE POLYMERS 332(2024):13.