Analysis of differentially expressed genes in the sea cucumber <i>Apostichopus japonicus</i> under heat stress

doi:10.1007/s13131-023-2196-4

	*Analysis of differentially expressed genes in the sea cucumber Apostichopus japonicus* under heat stress**
	Xu, Dongxue1 ; Zhang, Jingjing 2; Song, Wenqi 1; Sun, Lina 3; Liu, Ji 1; Gu, Yuanxue 1; Chen, Yanru 1; Xia, Bin 1
	2023-11-01
发表期刊	ACTA OCEANOLOGICA SINICA
ISSN	0253-505X
卷号	42 期号:11 页码:117-126
通讯作者	Xia, Bin([email protected])
摘要	The sea cucumber Apostichopus japonicus plays important roles in marine benthic ecosystem as environmental cleaners, and it is the important aquaculture species in China. High water temperature poses critical threat for the survival of A. japonicus, which has resulted in extensive death in summer. To explore the genes expression profiles under different levels of heat stress, the high-throughput RNA-seq was applied in this study. Our results revealed a total of 1 371, 1 225 and 1 408 differentially expressed genes (DEGs) in 26 degrees C for 6 h, 26 degrees C for 48 h and 30 degrees C for 6 h respectively in comparison with Control group. The pathway analysis suggested "Protein processing in endoplasmic reticulum (ER)" was significantly enriched in all these heat stress (HS) treatment groups. The expression results of key DEGs in this pathway (Hsp70, Derlin, NEF, PDI, GPR94 and ERP57) by qRT-PCR was in accordance with the RNA-seq data. The subcluster analysis of DEGs revealed that a variety of heat shock proteins (HSPs) and calcium ion binding proteins had an obvious up-regulated expression in 26 degrees C for 6 h, comparatively low expression in 26 degrees C for 48 h, and the highest expression in 30 degrees C for 6 h. The other DEGs subcluster, consisting of critical components of extracellular matrix (ECM) and a subset of peptidases and proteases, showed significantly rising tendency in 30 degrees C for 6 h. Additionally, the expression of matrix metalloproteases (MMP1, MMP16 and MMP19) was prominently affected by HS, and peaked in 30 degrees C for 6 h. This study provides a series of candidate genes for further study about heat shock response in A. japonicus, especially genes associated with protein processing in ER and regulation of ECM, which also offers new insights into cellular homeostasis under stressful conditions in marine invertebrates
关键词	Apostichopus japonicus heat stress RNA-seq protein processing extracellular matrix
DOI	10.1007/s13131-023-2196-4
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[42276143]; National Natural Science Foundation of China[31902360]; Shandong Provincial Natural Science Foundation[ZR2022MC050]; Shandong Provincial Natural Science Foundation[ZR2022QD003]; The First Class Fishery Discipline Programme in Shandong Province[(2020)3]
WOS研究方向	Oceanography
WOS类目	Oceanography
WOS记录号	WOS:001177284900001
出版者	SPRINGER
WOS关键词	EXTRACELLULAR-MATRIX ; STICHOPUS-JAPONICUS ; HIGH-TEMPERATURE ; IMMUNE-RESPONSE ; AUTOLYSIS ; HEAT-SHOCK-PROTEIN-70 ; METALLOPROTEINASES ; BIOMARKERS ; REGULATOR ; PROTEINS
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184918
专题	海洋生态与环境科学重点实验室
通讯作者	Xia, Bin
作者单位	1.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China 2.Ocean Univ China, Key Lab Mariculture, Minist Educ, Qingdao 266003, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Xu, Dongxue,Zhang, Jingjing,Song, Wenqi,et al. Analysis of differentially expressed genes in the sea cucumber Apostichopus japonicus under heat stress[J]. ACTA OCEANOLOGICA SINICA,2023,42(11):117-126.
APA	Xu, Dongxue.,Zhang, Jingjing.,Song, Wenqi.,Sun, Lina.,Liu, Ji.,...&Xia, Bin.(2023).Analysis of differentially expressed genes in the sea cucumber Apostichopus japonicus under heat stress.ACTA OCEANOLOGICA SINICA,42(11),117-126.
MLA	Xu, Dongxue,et al."Analysis of differentially expressed genes in the sea cucumber Apostichopus japonicus under heat stress".ACTA OCEANOLOGICA SINICA 42.11(2023):117-126.