Potential synergistic regulation of hsp70 and antioxidant enzyme genes in Pyropia yezoensis under high temperature stress

doi:10.1016/j.algal.2023.103375

IOCAS-IR > 实验海洋生物学重点实验室

	Potential synergistic regulation of hsp70 and antioxidant enzyme genes in Pyropia yezoensis under high temperature stress
	Huang, Danlin 1,2,5; Tian, Chao 1,2,5; Sun, Zhenjie 1,2,5; Niu, Jianfeng 1,2; Wang, Guangce 1,2,3,4
	2024-03-01
发表期刊	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN	2211-9264
卷号	78 页码:11
通讯作者	Niu, Jianfeng([email protected]) ; Wang, Guangce([email protected])
摘要	High temperatures have been regarded as one of the main factors limiting Pyropia yezoensis cultivation in recent years. Both the heat shock proteins and antioxidant enzymes genes were activated timely before heat damage to the cell. Here, the activities of different antioxidant enzymes under different conditions, including HSF inhibitor, CaM inhibitor, and HSP70 protein inhibitor were determined. Then, RNA-seq was performed using samples that had been pretreated with corresponding chemicals under high temperature conditions. The principal component analysis inferred that the shift of gene transcription patterns under high temperature conditions was closely related to HSF since the addition of QR suppressed the changes in gene transcription pattern. The hsp70 genes and antioxidant enzyme genes were categorized into three groups by the method of WGCNA. And the HSE sites were identified using plantPAN and FIMO in most genes of the categories showed relationship to HSF. Quantitative RT-PCR analysis of designated genes (FMPK >10) indicated that the expression of hsp70 and antioxidant enzyme genes in Py. yezoensis showed various transcriptional patterns, including upregulation, downregulation, and no significant changes in response to high temperature stress. The classification between qRT-PCR and WGCNA results showed a certain consistency. Considered the results above together, it was speculated that there were different HSFs regulated the expression of different genes of hsp70 and antioxidant enzymes, and HSP70 might also regulate the expression of anti-oxidase genes under the conditions of long-term stress. Additionally, transcription factors other than HSF might be involved in the high temperature stress response. The results indicated the complexity of heat stress response mechanisms in Py. yezoensis.
关键词	High temperatures Antioxidant enzymes hsp70 Heat shock transcription factor Pyropia yezoensis
DOI	10.1016/j.algal.2023.103375
收录类别	SCI
语种	英语
资助项目	Major Scientific and Technological Innovation Project of Shandong Provincial Key Research and Development Program[2022LZGC004]; National Natural Science Foundation of China[42376091]; China Agriculture Research System of MOF and MARA[CARS -50]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:001174430400001
出版者	ELSEVIER
WOS关键词	HEAT-SHOCK FACTOR ; TRANSCRIPTION FACTORS ; MOLECULAR CHAPERONES ; EXPRESSION ; TOLERANCE ; HSP70 ; ARABIDOPSIS ; BINDING ; HSF1 ; PROTEIN
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184897
专题	实验海洋生物学重点实验室
通讯作者	Niu, Jianfeng; Wang, Guangce
作者单位	1.Chinese Acad Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao, Peoples R China 4.Laoshan Lab, Qingdao, Peoples R China 5.Qingdao Agr Univ, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Huang, Danlin,Tian, Chao,Sun, Zhenjie,et al. Potential synergistic regulation of hsp70 and antioxidant enzyme genes in Pyropia yezoensis under high temperature stress[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2024,78:11.
APA	Huang, Danlin,Tian, Chao,Sun, Zhenjie,Niu, Jianfeng,&Wang, Guangce.(2024).Potential synergistic regulation of hsp70 and antioxidant enzyme genes in Pyropia yezoensis under high temperature stress.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,78,11.
MLA	Huang, Danlin,et al."Potential synergistic regulation of hsp70 and antioxidant enzyme genes in Pyropia yezoensis under high temperature stress".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 78(2024):11.