Proteomic insights into the response of <i>Halomonas</i> sp. MNB13 to excess Mn (II) and the role of H<sub>2</sub>S in Mn(II) resistance

doi:10.1016/j.envres.2024.118157

	*Proteomic insights into the response of Halomonas* sp. MNB13 to excess Mn (II) and the role of H₂S in Mn(II) resistance**
	Xu, Xiuli 1; Song, Fuhang 2; Zhang, Guoliang 3; Ma, Linlin 4; Yang, Na 5,6
	2024-04-01
发表期刊	ENVIRONMENTAL RESEARCH
ISSN	0013-9351
卷号	246 页码:10
通讯作者	Yang, Na([email protected])
摘要	Halomonas spp. are moderately halophilic bacteria with the ability to tolerate various heavy metals. However, the role of basic cellular metabolism, particularly amino acid metabolism, has not been investigated in Halomonas spp. under excess Mn(II). The strain Halomonas sp. MNB13 was isolated from a deep-sea ferromanganese nodule and can tolerate 80 mM Mn(II). To comprehensively explore the mechanisms underlying its resistance to excess Mn(II), we conducted a comparative proteome analysis. The data revealed that both 10 mM and 50 mM Mn(II) significantly up-regulated the expression of proteins involved in Mn(II) transport (MntE), oxidative stress response (alkyl hydroperoxide reductase and the Suf system), and amino acid metabolism (arginine, cysteine, methionine, and phenylalanine). We further investigated the role of cysteine metabolism in Mn(II) resistance by examining the function of its downstream product, H2S. Consistent with the up-regulation of cysteine desulfurase, we detected an elevated level of H2S in Halomonas sp. MNB13 cells under Mn(II) stress, along with increased intracellular levels of H2O2 and O-2(center dot-). Upon exogenous addition of H2S, we observed a significant restoration of the growth of Halomonas sp. MNB13. Moreover, we identified decreased intracellular levels of H2O2 and O-2(center dot-) in MNB13 cells, which coincided with a decreased formation of Mn-oxides during cultivation. In contrast, in cultures containing NaHS, the residual Mn(II) levels were higher than in cultures without NaHS. Therefore, H2S improves Mn(II) tolerance by eliminating intracellular reactive oxygen species rather than decreasing Mn(II) concentration in solution. Our findings indicate that cysteine metabolism, particularly the intermediate H2S, plays a pivotal role in Mn(II) resistance by mitigating the damage caused by reactive oxygen species. These findings provide new insights into the amino acid mechanisms associated with Mn(II) resistance in bacteria.
关键词	Halomonas sp. MNB13 Proteomic analysis Mn(II) resistance Cysteine metabolism H2S Reactive oxygen species
DOI	10.1016/j.envres.2024.118157
收录类别	SCI
语种	英语
资助项目	Qingdao Natural Science Foundation, China[23-2-1-174-zyyd-jch]; Strategic Priority Research Program of the Chinese Academy of Sciences, China[XDB42000000]; Research Foundation for Advanced Talents of Beijing Technology and Business University[19008020158]
WOS研究方向	Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
WOS类目	Environmental Sciences ; Public, Environmental & Occupational Health
WOS记录号	WOS:001161956800001
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS关键词	PHENYLALANINE AMMONIA-LYASE ; HYDROGEN-SULFIDE ; OXIDATIVE STRESS ; PLANT-RESPONSES ; CYSTEINE CYCLE ; BACTERIUM ; TOLERANCE ; MECHANISM ; TOXICITY ; CADMIUM
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184581
专题	深海极端环境与生命过程研究中心实验海洋生物学重点实验室
通讯作者	Yang, Na
作者单位	1.China Univ Geosci, Sch Ocean Sci, 29 Xueyuan Rd, Beijing 100083, Peoples R China 2.Beijing Technol & Business Univ, Sch Light Ind, 11 Fucheng Rd, Beijing 100048, Peoples R China 3.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Griffith Univ, Griffith Inst Drug Discovery, Sch Environm & Sci, Brisbane 4111, Australia 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 6.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266237, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Xu, Xiuli,Song, Fuhang,Zhang, Guoliang,et al. Proteomic insights into the response of Halomonas sp. MNB13 to excess Mn (II) and the role of H₂S in Mn(II) resistance[J]. ENVIRONMENTAL RESEARCH,2024,246:10.
APA	Xu, Xiuli,Song, Fuhang,Zhang, Guoliang,Ma, Linlin,&Yang, Na.(2024).Proteomic insights into the response of Halomonas sp. MNB13 to excess Mn (II) and the role of H₂S in Mn(II) resistance.ENVIRONMENTAL RESEARCH,246,10.
MLA	Xu, Xiuli,et al."Proteomic insights into the response of Halomonas sp. MNB13 to excess Mn (II) and the role of H₂S in Mn(II) resistance".ENVIRONMENTAL RESEARCH 246(2024):10.