Molecular and physiological responses in the ammonia transport pathways in clam<i> Cyclina</i><i> sinensis</i> exposed to chronic ammonia nitrogen

doi:10.1016/j.aqrep.2024.101952

IOCAS-IR

	Molecular and physiological responses in the ammonia transport pathways in clam Cyclina sinensis exposed to chronic ammonia nitrogen
	Ni, Qian 1; Liang, Xiafei 1; Yang, Shengyi 1; Ge, Hongxing 1,2,4; Dong, Zhiguo 1,2,3
	2024-04-01
发表期刊	AQUACULTURE REPORTS
ISSN	2352-5134
卷号	35 页码:9
通讯作者	Ge, Hongxing([email protected]) ; Dong, Zhiguo([email protected])
摘要	The clam Cyclina sinensis is characterized by good ammonia resistance. With the purpose of gaining knowledge about its ammonia resistance, we assessed the mRNA expression levels of Rhesus glucoprotein b (CsRhbg), the activities of key enzymes in the ammonia transport pathways (glutamine synthetase, GS; glutamic acid dehydrogenase, GDH) in the gill and hepatopancreas and the gill histopathological section (HE staining) of the clam exposed to total ammonia nitrogen (TAN) (6.58 mg/L) for 768 h (32d). Results revealed that the full-length cDNA sequence of CsRhbg was 1777 bp in length. The CsRhbg was highly conserved in the same genus, and the protein encoded by CsRhbg contains 10 transmembrane structures (TMHs) and one signal peptide. Exposed to ammonia nitrogen, the expression level of CsRhbg mRNA climbed sharply at 3 h post-exposure, and then it decreased and fluctuated from 192 h to 768 h. The activity of GSase in the clam exposed to ammonia nitrogen increased in the first 24 h, and reached the highest value at 96 h, and then fluctuated. The activity of GDH rose sharply and reached the highest value at 3 h, and then fluctuated. With the increase of exposure time, histological alterations in the gill became more and more clear in the disappearance of the epithelial cell boundaries. The results suggest that exposure to ammonia nitrogen can activate CsRhbg gene expression, and elevate the GSase and GDH activity in the gill of the clam as the ammonia detoxification strategy to reduce the harm of ammonia. However, prolonged exposure to ammonia nitrogen can result in the down-regulation of CsRhbg gene, dysfunction of key enzymes in the ammonia transport pathways, and damage to the structure of epithelial cells or the cellular structure in the gill of the clam. Therefore, this work will help to reveal the ammonia detoxification strategy in bivalves exposed to chronic ammonia nitrogen.
关键词	CsRhbg GSase GDH activity Cyclina sinensis Chronic ammonia nitrogen exposure
DOI	10.1016/j.aqrep.2024.101952
收录类别	SCI
语种	英语
资助项目	China Agriculture Research System of MOF; Postdoctoral Fellowship of Lianyungang[SH20211209]; Opening Foundation of Jiangsu Key Laboratory of Marine Bioresources and Environment[JSIMR202017]; Opening Foundation of Jiangsu Institute of Marine Resources Development[JBGS [2021] 034]; JBGS Project of Seed Industry Revitalization in Jiangsu Province[CARS-49]; China Agriculture Research System of MARA; Priority Academic Program Development of Jiangsu; [LYG20210008]
WOS研究方向	Fisheries
WOS类目	Fisheries
WOS记录号	WOS:001173451900001
出版者	ELSEVIER
WOS关键词	RAINBOW-TROUT ; GLUTAMINE-SYNTHETASE ; RHESUS GLYCOPROTEINS ; SUBLETHAL AMMONIA ; EXPRESSION ; BRAIN ; INHIBITION ; METABOLISM ; EXCRETION ; FAMILY
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184578
专题	中国科学院海洋研究所
通讯作者	Ge, Hongxing; Dong, Zhiguo
作者单位	1.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Bioresources & Environm, Lianyungang 222005, Jiangsu, Peoples R China 2.Jiangsu Marine Resources Dev Inst, Lianyungang 222005, Jiangsu, Peoples R China 3.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Jiangsu, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Ni, Qian,Liang, Xiafei,Yang, Shengyi,et al. Molecular and physiological responses in the ammonia transport pathways in clam Cyclina sinensis exposed to chronic ammonia nitrogen[J]. AQUACULTURE REPORTS,2024,35:9.
APA	Ni, Qian,Liang, Xiafei,Yang, Shengyi,Ge, Hongxing,&Dong, Zhiguo.(2024).Molecular and physiological responses in the ammonia transport pathways in clam Cyclina sinensis exposed to chronic ammonia nitrogen.AQUACULTURE REPORTS,35,9.
MLA	Ni, Qian,et al."Molecular and physiological responses in the ammonia transport pathways in clam Cyclina sinensis exposed to chronic ammonia nitrogen".AQUACULTURE REPORTS 35(2024):9.