A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates

doi:10.1021/acs.jafc.3c08638

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	A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates
	Gu, Xiaoqian 1,2; Fu, Liping 1; Wang, Zhiyan 1; Cao, Zhe 1; Zhao, Luying 1; Seswita-Zilda, Dewi 3; Zhang, Ao 4; Zhang, Qian 1; Li, Jiang 1
	2024-02-19
发表期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN	0021-8561
卷号	72 期号:8 页码:4116-4126
通讯作者	Li, Jiang([email protected])
摘要	Alginate lyase Aly448, a potential new member of the polysaccharide lyase (PL) 7 family, which was cloned and identified from the macroalgae-associated bacterial metagenomic library, showed bifunctionality. The molecular docking results revealed that Aly448 has two completely different binding sites for alginate (polyMG), poly-alpha-l-guluronic acid (polyG), and poly-beta-d-mannuronic acid (polyM) substrates, respectively, which might be the molecular basis for the enzyme's bifunctionality. Truncational results confirmed that predicted key residues affected the bifunctionality of Aly448, but did not wholly explain. Besides, Aly448 presented excellent biochemical characteristics, such as higher thermal stability and pH tolerance. Degradation of polyMG, polyM, and polyG substrates by Aly448 produced tetrasaccharide (DP4), disaccharide (DP2), and galactose (DP1), which exhibited excellent antioxidant activity. These findings provide novel insights into the substrate recognition mechanism of bifunctional alginate lyases and pave a new path for the exploitation of natural antioxidant agents.
关键词	bifunctional alginatelyase alginate oligosaccharides antioxidant activity
DOI	10.1021/acs.jafc.3c08638
收录类别	SCI
语种	英语
资助项目	Impact and Response of Antarctic Seas to Climate Change[IRFSOCC2023-2025]; Impact and Response of Antarctic Seas to Climate Change
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
WOS类目	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS记录号	WOS:001166898100001
出版者	AMER CHEMICAL SOC
WOS关键词	PURIFICATION ; OLIGOSACCHARIDES ; PL-7
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184488
专题	实验海洋生物学重点实验室
通讯作者	Li, Jiang
作者单位	1.Minist Nat Resources, Inst Oceanog 1, Key Lab Ecol Environm Sci & Technol, Qingdao 266061, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Natl Res & Innovat Agcy BRIN, Res Ctr Deep Sea Earth Sci & Maritime Res Org, Jakarta 14430, Indonesia 4.Qingdao Univ Sci & Technol, Chem Engn Inst, Qingdao 266042, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Gu, Xiaoqian,Fu, Liping,Wang, Zhiyan,et al. A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2024,72(8):4116-4126.
APA	Gu, Xiaoqian.,Fu, Liping.,Wang, Zhiyan.,Cao, Zhe.,Zhao, Luying.,...&Li, Jiang.(2024).A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,72(8),4116-4126.
MLA	Gu, Xiaoqian,et al."A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72.8(2024):4116-4126.